Use of sequence characterised amplified region and RAPD markers in the identification of the white truffle Tuber magnatum Pico |
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Authors: | Amicucci Antonella Rossi Ismaela Potenza Lucia Agostini Deborah Stocchi Vilberto |
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Institution: | (1) Istituto di Chimica Biologica, Università degli Studi di Urbino, Via Saffi, 2-61029 Urbino (PS), Italy |
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Abstract: | Isolates of white truffles were identified as Tuber magnatum Pico species using a pair of primers selected from a sequence characterised amplified region (SCAR) and a specific random amplified polymorphic DNA (RAPD) marker. The present study reveals that PCR-fragment-pattern polymorphisms, the construction of probes and couples of primers from one or more of these polymorphic fragments may provide a useful and rapid tool for identifying species of ectomycorrhizal fungi in addition to conventional methods (morphological parameters). |
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