Rapid Quantitative Detection of Listeria monocytogenes in Meat Products by Real-Time PCR |
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| Authors: | David Rodrí guez-L zaro, Anna Jofr , Teresa Aymerich, Marta Hugas, Maria Pla |
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| Affiliation: | David Rodríguez-Lázaro, Anna Jofré, Teresa Aymerich, Marta Hugas, and Maria Pla |
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| Abstract: | We describe a quick and simple method for the quantitative detection of Listeria monocytogenes in meat products. This method is based on filtration, Chelex-100-based DNA purification, and real-time PCR. It can detect as few as 100 CFU/g and quantify as few as 1,000 CFU/g, with excellent accuracy compared to that of the plate count method. Therefore, it is a promising alternative for the detection of L. monocytogenes in meat products. |
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