A novel alkaline, thermostable, protease-free lipase from Pseudomonas sp. |
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Authors: | Neelima Kulkarni RV Gadre |
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Institution: | (1) Chemical Engineering Division, National Chemical Laboratory, Pune, 411008, India |
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Abstract: | An extracellular, alkali-tolerant, thermostable lipase was from a Pseudomonas sp. It had optimal activity at 65 °C and retained 75% of its activity at 65 °C for 90 min. The pH optimum was 9.6 and it retained more than 70% activity between pH 5 and 9 for 2 h. The culture broth was free of protease and, at 30 °C, the culture filtrate retained all the activity for at least 7 days, without any stabilizer. In shake flask culture, addition of groundnut oil (3 g l–1) towards the end of growth phase increased the activity from 4 U ml–1 to 8 ml–1. |
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Keywords: | alkali-tolerant lipase protease-free Pseudomonas thermostable |
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