| 大豆GmPDS基因的克隆及VIGS表达载体构建和鉴定 |
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| 引用本文： | 吕山花,樊颖伦,吕福堂,刘立科,孙亚梅,马姗姗.大豆GmPDS基因的克隆及VIGS表达载体构建和鉴定[J].生物技术通报,2010(4). |
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| 作者姓名： | 吕山花 樊颖伦 吕福堂 刘立科 孙亚梅 马姗姗 |
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| 作者单位： | 1. 聊城大学农学院,聊城,252059
2. 聊城大学生命科学院,聊城,252059 |
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| 基金项目： | 聊城大学博士科研启动基金 |
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| 摘 要： | 病毒诱导的基因沉默(virus induced gene silencing,VIGS)技术是近年来发展起来的一种反向遗传学快速研究基因功能的方法,对于植物特别是难于转化的大豆而言尤其适用。本研究采用PCR技术从大豆(Glycine max)基因组中克隆了八氢番茄红素去饱和酶(phytoene desaturase,PDS)基因的部分序列,命名为GmPDS(Glycinemax PDS)。该片段长430bp,序列分析表明,该基因与大豆八氢番茄红素去饱和酶(GenBank登录号:M64704)cDNA序列同源性为99%。双酶切GmPDS片段和烟草脆裂病毒载体(pTRV2),构建了重组载体pTRV2-GmPDS,并将该重组载体分别转化农杆菌C58C1/pMP90、GV3101和LBA4404,为分析pTRV载体是否可以浸染大豆奠定了基础。
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| 关 键 词： | 大豆 GmPDS基因 载体构建 |
Construction and Identification of VIGS Expression Vector Carrying GmPDS Gene Cloned from Glycine max |
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| Lv Shanhua,Fan Yinglun,Lv Futang,Liu Like,Sun Yamei,Ma Shanshan.Construction and Identification of VIGS Expression Vector Carrying GmPDS Gene Cloned from Glycine max[J].Biotechnology Bulletin,2010(4). |
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| Authors: | Lv Shanhua Fan Yinglun Lv Futang Liu Like Sun Yamei Ma Shanshan |
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| Institution: | Lv Shanhua1Fan Yinglun1Lv Futang1Liu Like2Sun Yamei1Ma Shanshan1(1School of Agriculture,Liaocheng University,Liaocheng 252059,2School of Life Science,Liaocheng 252059 ) |
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| Abstract: | Virus induced gene silencing (VIGS) is increasingly being used as a reverse genetics tool to study functions of specific plant genes in a short time. It is especially useful for plants,especially soybean that is recalcitrant to transformation. A partial sequence of phytoene desaturase (PDS)was cloned from Glycine max using PCR method and termed as GmPDS (Glycine max PDS). GmPDS indicates 430 bp length,and exhibits 99% identity to the cDNA sequences that PDS was obtained from GenBank database (GenBank access... |
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| Keywords: | VIGS Glycine max GmPDS gene VIGS Vector construction |
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