Allelic loss at PTEN locus leads to progression of colorectal carcinoma among North Indian patients |
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| Authors: | Asgar Ali Syed Jafar Mehdi Krishnan Hajela Sundeep Singh Saluja Pramod Kumar Mishra Aga Syed Sameer |
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| Affiliation: | 1. Department of Biochemistry, AIIMS, Patna, India;2. Department of Biosciences, Genome Biology Lab, Jamia Millia Islamia, New Delhi, India;3. School of Life Sciences, Devi Ahilya Vishwavidyalaya, Indore, India;4. Department of Gastrointestinal Surgery, G. B. Pant Hospital, New Delhi, India;5. Basic Medical Sciences, College of Medicine-Jeddah, King Saud bin Abdulaziz University for Health Sciences, Riyadh, Saudi Arabia |
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| Abstract: | We evaluated the loss of heterozygosity (LOH) at 10q23.3 locus of microsatellite markers; D10S198, D10S192, and D10S541 of PTEN gene in 223 North Indian colorectal cancer (CRC) specimens. DNA was isolated and microsatellite-specific markers polymerase chain reaction was performed. Out of total 223 cases 102 showed LOH for at least one of the locus. In addition, thereto a significant association was found with the clinicopathologic features like grade of differentiation, clinical stage, invasion, lymph node invasion, and the clinical outcome (p?PTEN gene at locus 10q23.3 could be considered as one of the diagnostic markers in CRC. |
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| Keywords: | Colorectal cancer loss of heterozygosity microsatellite marker PCR PTEN |
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