|
|||||
|
|
| 不同材质微芯片内细胞生长状态分析 | |
| 引用本文： | 邵建波,吴蕾,赵辉,郑允焕,吴建璋,金庆辉,赵建龙.不同材质微芯片内细胞生长状态分析[J].生物物理学报,2008,24(4):268-272. |
| 作者姓名： | 邵建波 吴蕾 赵辉 郑允焕 吴建璋 金庆辉 赵建龙 |
| 作者单位： | 1. 中国科学院上海微系统与信息技术研究所,上海,200050;中国科学院研究生院,北京,100039 2. 中国科学院上海微系统与信息技术研究所,上海,200050 |
| 基金项目： | 国家自然科学基金项目（30771115），上海市科委项目（0652nm016, 06JC14081, 0752nm021, 06xd14037），973 项目（2005CB724305） |
| 摘 要： | 微系统技术在细胞生物学方面的研究中已得到广泛应用。了解细胞在微系统芯片内的生长状态，对于利用微系统技术进行细胞研究有重要的指导意义。玻璃和聚二甲基硅氧烷（polydimethylsiloxane，PDMS）是目前制作细胞培养微芯片的主要材料。通过向以二者为基底材料制作的细胞培养微芯片内导入内皮细胞进行培养，利用实验室构建的细胞成像分析系统观察和分析细胞在不同基底材料的芯片内5天的增殖情况，同时研究了基底材料的预处理方法以及培养基对细胞增殖的影响。 |
| 关 键 词： | 细胞培养 玻璃 聚二甲基硅氧烷 微芯片 增殖速率 |
| 收稿时间： | 2007-11-23 |
CELL VIABILITY STUDY IN MICROCHIP OF DIFFERENT MATERIALS |
|
| SHAO Jian-bo,WU Lei,ZHAO Hui,ZHENG Yun-huan,WU Jian-zhang,JIN Qing-hui,ZHO Jian-long.CELL VIABILITY STUDY IN MICROCHIP OF DIFFERENT MATERIALS[J].Acta Biophysica Sinica,2008,24(4):268-272. | |
| Authors: | SHAO Jian-bo WU Lei ZHAO Hui ZHENG Yun-huan WU Jian-zhang JIN Qing-hui ZHO Jian-long |
| Institution: | 1. Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Science, Shanghai 200050, China; 2. Graduate School of the Chinese Academy of Science, Beijing 100049, China |
| Abstract: | Microsystem technologies have widely applied in cell biology study. Understanding the viability of cells in microsystem is important to direct the cell research by the way. Glass and polydimethylsiloxane are familiar in the fabrications of microchip for cell research.. They were used to bond with PDMS to make cell culture chip for cell viability study. The endothelial cells were infused in the microchip and their growth in five days were observed. We also studied the influences of the treatment of chips before cell infused and the changes of culture medium. |
| Keywords: | Cell culture Glass PDMS Microchip Grow rate |
| 本文献已被 维普 万方数据 等数据库收录！ | |
| 点击此处可从《生物物理学报》浏览原始摘要信息 | |
| 点击此处可从《生物物理学报》下载免费的PDF全文 | |