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Cryopreservation of |
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| Authors: | H?Verleysen |
| Affiliation: | (1) Department of Plant Production, Faculty of Bioscience Engineering, University Gent, Coupure links 653, 9000 Gent, Belgium;(2) Department of Plant Genetics and Breeding, CLO Gent, Caritasstraat 21, Melle, 9090 , Belgium |
| Abstract: | Cryopreservation of Robinia pseudoacacia explants by vitrification achieved 78% survival following the stepwise preculture of shoot tips in (0.3 + 0.5 + 0.7 M) sucrose with a 80 min incubation in PVS2; compared to 87% survival after desiccation of explants to 30% water content, following 3 days alginate bead (with glycerol and sucrose treatments) preculture in 0.7 M sucrose. |
| Keywords: | alginate black locust encapsulation-dehydration glycerol PVS2 Robinia pseudoacacia sucrose vitrification |
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