Identification of <Emphasis Type="Italic">Porphyra yezoensis</Emphasis> (Rhodophyta) meiosis by DNA quantification using confocal laser scanning microscopy |
| |
Authors: | Akio Shimizu Kagayaki Morishima Masahiro Kobayashi Masahiko Kunimoto Ichiro Nakayama |
| |
Institution: | (1) National Research Institute of Fisheries Science, Fisheries Research Agency, Fukuura 2-12-4, Kanazawa Yokohama, 236-8648, Japan;(2) Present address: Graduate School of Fisheries Science, Hokkaido University, Minato Hakodate, 014-8611, Japan;(3) Present address: Project Planning Department, Fisheries Research Agency, Minatomirai 2-3-3, Nishi-ku Yokohama, 220-6115, Japan |
| |
Abstract: | Conchospore germlings of Porphyra yezoensis were stained with a fluorescent dye for DNA and observed with confocal laser scanning microscopy (CLSM). Relative DNA values
of the germling nuclei were obtained by measuring fluorescence intensities of nuclear regions of the optically sliced specimens,
using the mean value of the smallest blade cells as a reference of the genomic n value. Such quantification revealed that the nuclear DNA amounts of the one-cell, two-cell, and four-cell-stage germlings
are approximately 4 × n, 2 × n, and n ∼2 × n values respectively; these values agreed well with the expected ones from the hypothesis that meiosis corresponds to the
first successive cell divisions after the conchospore germination. These results are consistent with a previous study on cytogenetic
analysis of the chimaera blade formation (Ohme and Miura 1988, Plant Sci 57:135–140) and not consistent with a recent microscopic study (Wang et al. 2006, Phycol Res 54:201–207) which proposed that the first meiotic division occurs at the conchospore formation and the second
division at the germination. |
| |
Keywords: | Porphyra Red algae Confocal microscopy DNA quantification Conchospore germling Meiosis Nuclear phase |
本文献已被 SpringerLink 等数据库收录! |
|