| 白菜转脂蛋白CaMBP10分子中钙调素结合结构域的鉴定 |
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| 引用本文： | 金犂天,胖铁良,李振鹏,谢万钦,李翠凤.白菜转脂蛋白CaMBP10分子中钙调素结合结构域的鉴定[J].中国生物化学与分子生物学报,2011,27(10):942-949. |
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| 作者姓名： | 金犂天 胖铁良 李振鹏 谢万钦 李翠凤 |
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| 作者单位： | 南开大学生命科学学院生物化学与分子生物学系; |
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| 基金项目： | 国家自然科学基金(No.30871614); 天津市自然科学基金(No.08JCYBJC04100)资助项目~~ |
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| 摘 要： | 植物转脂蛋白(LTPs)是多基因编码的蛋白家族, 广泛分布于高等植物，其确切的生理功能至今仍不清楚. 本室从白菜中分离的钙调素结合蛋白-10 (CaMBP10) 经序列分析 被鉴定为植物转脂蛋白家族成员,体外实验证明钙调素(CaM)调节其脂质结合活性.为了深入了解转脂蛋白与CaM的相互作用机制，本文通过删除、缺失和定点突变等分子生物学手段确定了白菜转脂蛋白CaMBP10分子中的钙调素结合结构域.该结构域位于分子C末端 64~83位氨基酸残基之间，其中疏水氨基酸的分布具有1-5-8-10 的CaM结合模序特征.
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| 关 键 词： | 植物转脂蛋白 钙调素结合蛋白 钙调素结合结构域 脂质结合活性 |
| 收稿时间： | 2011-07-14 |
Identification of CaM-binding Domain of Cabbage Lipid Transfer Protein CaMBP10 |
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| JIN Li-Tian,PANG Tie-Liang,LI Zhen-Peng,XIE Wan-Qin,LI Cui-Feng.Identification of CaM-binding Domain of Cabbage Lipid Transfer Protein CaMBP10[J].Chinese Journal of Biochemistry and Molecular Biology,2011,27(10):942-949. |
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| Authors: | JIN Li-Tian PANG Tie-Liang LI Zhen-Peng XIE Wan-Qin LI Cui-Feng |
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| Institution: | JIN Li-Tian,PANG Tie-Liang,LI Zhen-Peng,XIE Wan-Qin,LI Cui-Feng(Department of Biochemistry and Molecular Biology,Nankai University,Tianjin 300071,China) |
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| Abstract: | Plant non-specific lipid transfer proteins(ns-LTPs) encoded by multigene families are distributed ubiquitously throughout the plant kingdom.Their biological functions in vivo remain unclear.Recently,it has been proposed that ns-LTPs may play a key role in plant defense mechanisms,particularly during the induction of systemic acquired resistance.However,very little was known about the regulation in this process.A CaM-binding protein-10(CaMBP10) isolated from Chinese cabbage,is identified as a new member of l... |
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| Keywords: | plant lipid transfer protein calmodulin-binding protein calmodulin-binding domain lipid-binding activity |
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