| 2型糖尿病小鼠食道菌群的结构分析 |
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| 引用本文： | 胡芳,魏婷婷,杜彩贺,周东蕊,张仁敏,张红琳,陆祖宏.2型糖尿病小鼠食道菌群的结构分析[J].中国生物化学与分子生物学报,2011,27(10):972-979. |
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| 作者姓名： | 胡芳 魏婷婷 杜彩贺 周东蕊 张仁敏 张红琳 陆祖宏 |
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| 作者单位： | 东南大学生物科学与医学工程学院;东南大学学习科学研究中心; |
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| 基金项目： | 国家自然科学基金资助项目(No.60701008); 江苏省科技支撑计划(No.BE2010675)~~ |
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| 摘 要： | 人体内庞大的微生物群体对人体有着巨大的影响.越来越多的数据表明,肠道菌群与肥胖症、糖尿病等代谢性疾病的发生密切相关.食道菌群结构对研究胃肠道及整个消化 系统菌群结构至关重要.针对10只2型糖尿病模型小鼠及10只正常对照小鼠食道样本，进行变性梯度凝胶电泳（DGGE）和克隆测序分析食道菌群结构.结果发现,实验组小鼠与对照组小鼠食道菌群多样性指数与丰富度指数存在显著差异，均匀度指数无显著差 异.说明正常小鼠较2型糖尿病小鼠食道菌群种类及数量较大，优势菌种类及相对含量相似.测序结果显示,正常小鼠食道内含乳杆菌属细菌，而患病小鼠食道内不含乳杆菌属细菌或含量极低.提示乳杆菌属细菌与2型糖尿病密切相关，实验结果对研究糖尿病发病机理及并发症治疗有重要意义.
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| 关 键 词： | 2型糖尿病 BKS-DB小鼠 食道菌群结构 变性梯度凝胶电泳（DGGE） |
| 收稿时间： | 2011-08-18 |
Variation Analysis of Bacterial Communities in Esophagus of BKS-DB Mice |
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| HU Fang,WEI Ting-Ting,DU Cai-He,ZHOU Dong-Rui,ZHANG Ren-Min,ZHANG Hong-Lin,LU Zu-Hong,School of Biological Science , Medical Engineering,Southeast University,Nanjing ,China,Research Center for Learning Science,China.Variation Analysis of Bacterial Communities in Esophagus of BKS-DB Mice[J].Chinese Journal of Biochemistry and Molecular Biology,2011,27(10):972-979. |
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| Authors: | HU Fang WEI Ting-Ting DU Cai-He ZHOU Dong-Rui ZHANG Ren-Min ZHANG Hong-Lin LU Zu-Hong School of Biological Science Medical Engineering Southeast University Nanjing China Research Center for Learning Science China |
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| Institution: | HU Fang1),WEI Ting-Ting2),DU Cai-He2),ZHOU Dong-Rui2),ZHANG Ren-Min2),ZHANG Hong-Lin2),LU Zu-Hong1),2)(1)School of Biological Science and Medical Engineering,Southeast University,Nanjing 210096,China,2)Research Center for Learning Science,China) |
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| Abstract: | The microbial communities great affect human physiology.Increasing data showed that the intestinal flora were closely related to metabolic disease,such as obesity and diabetes.Study of esophageal flora is crucial for investigations on microbial communities in gastrointestinal tract of the whole digestive system.In this study,esophageal samples of 10 BKS-DB mice and 10 normal control mice were collected.Analyses using PCR-DGGE and cloning sequencing to study the bacterial communities were performed.Comparing... |
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| Keywords: | type 2 diabetes BKS-DB mice esophageal flora DGGE |
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