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| 雪山豆凝集素红细胞凝集成分的纯化 | |
| 引用本文： | 丁邦裕. 雪山豆凝集素红细胞凝集成分的纯化[J]. 云南植物研究, 1987, 0(1) |
| 作者姓名： | 丁邦裕 |
| 作者单位： | 江苏省肿瘤防治研究所 南京 |
| 摘 要： | 雪山豆凝集素(SML)经SP-Sephadex C-50和磷酸纤维素离子交换层析分离红血球凝集成分。聚丙烯酰胺凝胶电泳分析和红血球凝集试验为一不均一成分。其中E_2-SML有两条迁移较快的蛋白带,红细胞凝集效价是SML的64倍,是L-SML的512倍。该法制备的红细胞凝集成分具有高的产量和纯度。 |
| 关 键 词： | 雪山豆凝集素 红细胞凝集素 |
PURIFICATION OF ERYTHROAGGLUTININ COMPONENTS FROM SNOW MOUNTAIN BEAN LECTIN |
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| Ding Bangyu. PURIFICATION OF ERYTHROAGGLUTININ COMPONENTS FROM SNOW MOUNTAIN BEAN LECTIN[J]. Acta Botanica Yunnanica, 1987, 0(1) | |
| Authors: | Ding Bangyu |
| Abstract: | Erythroagglutinin components (E2-SML) are purified from Snow Mountain Bean Lectin (SML) by ion exchange chromatography on SP-Sephadex C-50 and Phosphocellulose column. They are monitored by polyacrylamide gel electrophoresis analysis and hemagglutinating assays. The cathodal proteins (E2 -SML) are potent hemagglutinins with hemagglutination titers 64 and 512 times more potent than SML and L-SML respectively. The methods we used can separate active components of erythroagglutinin lectin in large yield and high purity. |
| Keywords: | Snow mountain bean lcctin Erythroagglutinin |
| 本文献已被 CNKI 等数据库收录！ | |