Changes in RACK1 expression induce defects in nodulation and development in Phaseolus vulgaris |
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Authors: | Tania Islas-Flores Gabriel Guillén Federico Sánchez Marco A Villanueva |
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Institution: | 1.Departamento de Biología Molecular de Plantas Instituto de Biotecnología Universidad Nacional Autónoma de México; U.N.A.M.; Morelos, México;2.Instituto de Ciencias del Mar y Limnología Unidad Académica de Sistemas Arrecifales Universidad Nacional Autónoma de México, U. N. A. M. Prolongación Avenida Niños Héroes S/N Puerto Morelos; Quintana Roo, México |
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Abstract: | RACK1 is a scaffold protein with the ability to interact in a regulated manner with a diverse number of ligands from distinct signal-transduction pathways. This assessment allowed us to infer that it may be involved in different processes such as nodulation. In a recent study we showed by silencing, that PvRACK1 has a pivotal role in cell expansion and in symbiosome and bacteroid integrity during nodule development in Phaseolus vulgaris. On the other hand, we have also observed that its overexpression provokes a dramatic phenotype in: (a) seedlings that have been exposed to heat, in which systemic necrosis is induced; and (b) in Agrobacterium rhizogenes-transformed roots, where nodulation is strongly inhibited and nodules show early senescent symptoms. These findings indicate that PvRACK1 may be an integrator of diverse signal-transduction pathways in processes as varied as nodulation, cell expansion, heat stress responses, and systemic activation of necrosis. |
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Keywords: | P vulgaris inhibition necrosis nodulation over-expression silencing systemic |
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