A leucine zipper-based peptide hybrid delivers functional Nanog protein inside the cell nucleus |
| |
| Authors: | Yoshiyuki Hakata Hiroyuki Michiue Takashi Ohtsuki Masaaki Miyazawa Mizuki Kitamatsu |
| |
| Institution: | 1. Department of Immunology, Faculty of Medicine, Kindai University, 377-2 Ohno-Higashi, Sayama, Osaka 589-8511, Japan;2. Neutron Therapy Research Center, Okayama University, 2-5-1 Shikata-cho, Kita-ku, Okayama 700-8558, Japan;3. Department of Biotechnology, Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University, 3-1-1 Tsushimanaka, Kita-ku, Okayama 700-8530, Japan;4. Department of Applied Chemistry, Faculty of Science and Engineering, Kindai University, 3-4-1 Kowakae, Higashi-Osaka, Osaka 577-8502, Japan |
| |
| Abstract: | We synthesized a pair of compounds containing leucine zipper peptides to deliver protein cargo into cells. One is a cell-penetrating peptide (CPP) with Lz(E), a leucine zipper peptide containing negatively charged amino acids, and the other is a Nanog protein with Lz(K), a leucine zipper peptide containing positively charged amino acids. When cells were treated with these equimolar mixtures, Nanog-Lz(K) hybridized with Lz(E)-CPP was successfully delivered into the cells. Furthermore, Nanog-Lz(K) exerted its proper function after nuclear transport. |
| |
| Keywords: | Peptide Cell-penetrating peptide Leucine zipper Nanog Drug delivery system |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
