| 多聚酶链反应技术鉴别肾综合征出血热病毒血清型的初步研究 |
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| 引用本文： | 汤一苇,Suyu L.Ruo,徐晓,Anthony Sanchez,Susan P.Fisher-Hoch,Joseph B.McCormick,徐志一.多聚酶链反应技术鉴别肾综合征出血热病毒血清型的初步研究[J].病毒学报,1990(4). |
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| 作者姓名： | 汤一苇 Suyu L.Ruo 徐晓 Anthony Sanchez Susan P.Fisher-Hoch Joseph B.McCormick 徐志一 |
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| 作者单位： | 上海医科大学公共卫生学院流行病学教研室
(汤一苇)，美国疾病控制中心病毒病研究所特别病原科
(Suyu L.Ruo，Anthony Sanchez，Susan P.Fisher-Hoch，Joseph B.McCormick)，浙江医科大学附属一院传染病学教研室
(徐晓)，上海医科大学公共卫生学院流行病学教研室(徐志一) |
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| 摘 要： | 肾综合征出血热(HFRS)为一组抗原性密切相关的布尼亚科汉坦病毒引起的急性传染病。在我国存在至少两种临床表现、动物宿主及流行特征截然不同的血清型别,即血清Ⅰ型(汉坦型)和血清Ⅱ型(汉城型)。这两型病毒间的血清学定型已有报道。近年来,除啮齿类动物外,从临床病人以及非啮齿类动物体内也分离到了HFRS病毒。同时出现两类型别毒株共存,以及从家鼠体内分离到野鼠型毒株或从野鼠体内分离到家鼠型毒株的复杂情形。为此,准确检定并鉴别不同来源毒株型别,将为深入了解其病原学、流行病学以及制定疫苗生产策略提供重要信息。
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| 关 键 词： | 肾综合征出血热病毒 多聚酶链反应 鉴别 |
A PRELIMINARY STUDY ON THE POLYMERASE CHAIN REACTION ASSAY FOR DIFFERENTIATION OF THE SEROTYPES OF HANTAVIRUSES |
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| Tang Yiwei Suyu L. Ruo Xu XiaoAnthony Sanchez Susan. P. Fisher-HochJoseph B. McCormick Xu Zhiyi.A PRELIMINARY STUDY ON THE POLYMERASE CHAIN REACTION ASSAY FOR DIFFERENTIATION OF THE SEROTYPES OF HANTAVIRUSES[J].Chinese Journal of Virology,1990(4). |
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| Authors: | Tang Yiwei Suyu L Ruo Xu XiaoAnthony Sanchez Susan P Fisher-HochJoseph B McCormick Xu Zhiyi |
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| Institution: | Tang Yiwei Suyu L. Ruo Xu XiaoAnthony Sanchez Susan. P. Fisher-HochJoseph B. McCormick Xu Zhiyi Department of Epidemiology,School of Public Health,Shanghai Medical University,ShanghaiDivision of Viral Diseases,Center for Infectious Diseases,Centers for Disease Control,Atlanta,USADepartment of Infectious Diseases,First Affiliated Hospital,Zhejiang Medical University,Hangzhou |
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| Abstract: | Seven Hantavirus strains isolated from different animal hosts and geographic regions were differentiated by polymerasc chain reaction ( PCR ) assay using the synthetic strain-specific oligoaucleotide primers designed with a region from 30 to 748 nucleotides of the M segment of HTN and R22 strains. 76-118-specific primers amplified only the RNAs extracted from Z20, J3 strains isolated from Apodemus agrarius in Zhejiang Province, HTN strain in Korea,and S2 strain from Suncus murinus in the suburbs of Shanghai. In contrast, R21-specific primers amplified only the RNAs extracted from R22, K24 strains isolated from Raitus norvegicus in Henan Province and SR11 strain in Japan. The identification of the generated DNA products was verified by dot-hybridization with internal probes specific for the M segment of HTN and R22 strains. As a new sensitive and specific molecular technique, PCR assay offers an opportunity to identify and differentiate the serotypes of Hantavirus strains in China quickly and accurately. |
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| Keywords: | Hantaviruses Polymerase chain reaction Differentiation |
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