| Folin-Ciocalteu比色法测定花椒中多酚含量的研究 |
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| 引用本文： | 吴亮亮,石雪萍,张卫明.Folin-Ciocalteu比色法测定花椒中多酚含量的研究[J].中国野生植物资源,2010,29(6):54-56. |
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| 作者姓名： | 吴亮亮 石雪萍 张卫明 |
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| 作者单位： | [1]南京野生植物综合利用研究院,江苏南京210042 [2]南京农业大学食品科技学院,江苏南京210095 |
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| 基金项目： | 国家"十一五"科技支撑计划重点课题 |
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| 摘 要： | 研究了以没食子酸为标准品,采用Folin-Ciocalteu比色法,测定花椒多酚含量的适宜条件。结果表明:当Folin-Ciocalteu试剂与10%Na2CO3溶液之比为1∶2(V∶V),Folin-Ciocalteu试剂体积为4 mL,反应温度为25℃,反应时间为120 min时,多酚含量在1~6μg/mL范围内与吸光值呈良好的线性关系。测定了4种花椒中的多酚含量,四川青花椒多酚含量最高。
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| 关 键 词： | 花椒 多酚 比色法 |
Study on Determination of Polyphenol Content in Zanthoxylum by Folin - Ciocalteu Colorimetry |
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| Wu Liangliang,Shi Xueping,Zhang Weiming.Study on Determination of Polyphenol Content in Zanthoxylum by Folin - Ciocalteu Colorimetry[J].Chinese Wild Plant Resources,2010,29(6):54-56. |
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| Authors: | Wu Liangliang Shi Xueping Zhang Weiming |
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| Institution: | 1.Nanjing Institute for Comprehensive Utilization of Wild Plant,Nanjing 210042,China;2.Nanjing Agriculture University,School of Food Science ＆ Technology,Nanjing 210095,China) |
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| Abstract: | Using gallic acid as the standard,the optimized conditions for determining content of phenol of Zanthoxylum by Folin-Ciocalteu Colorimetry.The results were as follows： volume ratio between Folin-Ciocalteu reagent and 10% Na2CO3 1∶2（V/V）,volume of Folin-Ciocalteu 4 mL,reaction temperature 25 ℃,and reaction time 120 min.There is a good linearity between content of phenol and absorbance in the range of 1~6 μg/mL.The content of polyphenols of four kinds of Zanthoxylum were determined.The highest content of polyphenol is Z.schinifolium from Sichuan. |
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| Keywords: | Zanthoxylum polyphenol colorimetry |
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