Factors regulating production of α-galactosidase from Bacillus sp. JF2 |
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| Authors: | X Li L Yang P Yan F Zuo & F Jin |
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| Institution: | Department of Food Technology, Dalian College of Light Industry, Dalian, China |
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| Abstract: | Certain factors affecting the production of cell-associated α-galactosidase by Bacillus sp. JF2 were investigated. The intention was to maximize α-galactosidase activity of potential commercial application, by consecutive optimization of growth media and conditions. The highest α-galactosidase activity was obtained when grown on melibiose, whereas sucrose inhibited the production of α-galactosidase. α-Galactosidase production was optimally active at pH 7·5 and 55°C. It was identified that a soy effluent stream could be used as the best carbon source for α-galactosidase by Bacillus sp. JF2. |
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