| 用于金刚石表面单分子有序固定的DNA折纸制备 |
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| 引用本文： | 魏一成,余佩,陈明,孙梓庭,曾俨,娄继忠,陈三友,石发展,杜江峰.用于金刚石表面单分子有序固定的DNA折纸制备[J].生物化学与生物物理进展,2020,47(1):53-60. |
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| 作者姓名： | 魏一成 余佩 陈明 孙梓庭 曾俨 娄继忠 陈三友 石发展 杜江峰 |
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| 作者单位： | 1)中国科学技术大学，合肥微尺度物质科学国家研究中心，合肥 230026;2)中国科学技术大学近代物理系和中国科学院微观磁共振重点实验室，合肥 230026,1)中国科学技术大学，合肥微尺度物质科学国家研究中心，合肥 230026;2)中国科学技术大学近代物理系和中国科学院微观磁共振重点实验室，合肥 230026,1)中国科学技术大学，合肥微尺度物质科学国家研究中心，合肥 230026;2)中国科学技术大学近代物理系和中国科学院微观磁共振重点实验室，合肥 230026,1)中国科学技术大学，合肥微尺度物质科学国家研究中心，合肥 230026;2)中国科学技术大学近代物理系和中国科学院微观磁共振重点实验室，合肥 230026,3)中国科学院生物物理研究所，中国科学院核酸生物学重点实验室，北京 100101,3)中国科学院生物物理研究所，中国科学院核酸生物学重点实验室，北京 100101,1)中国科学技术大学，合肥微尺度物质科学国家研究中心，合肥 230026;2)中国科学技术大学近代物理系和中国科学院微观磁共振重点实验室，合肥 230026,1)中国科学技术大学，合肥微尺度物质科学国家研究中心，合肥 230026;2)中国科学技术大学近代物理系和中国科学院微观磁共振重点实验室，合肥 230026,1)中国科学技术大学，合肥微尺度物质科学国家研究中心，合肥 230026;2)中国科学技术大学近代物理系和中国科学院微观磁共振重点实验室，合肥 230026 |
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| 基金项目： | 国家重点研发计划(2016YFA0502400)，国家自然科学基金(81788101, 31600685)资助项目. |
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| 摘 要： | 近年来兴起的以金刚石氮-空位(NV)色心为量子传感器的微观磁共振技术得到快速发展,已经实现单个生物分子磁共振谱的探测,正在向单分子结构和功能的研究推进.这其中需要解决一个重要的技术问题,即单分子在金刚石表面的有序分散和固定. DNA的自组装为解决这一问题提供了可行途径,本文使用DNA折纸技术,制备了一种60 nm边长的正方形双层DNA折纸作为单分子载体,并与金刚石表面结合.首先采用双层结构提高了DNA折纸的稳定性,其次通过在DNA折纸边缘添加发卡结构减少了DNA折纸结构间的聚团,最终成功将DNA折纸装配到金刚石表面.通过原子力显微镜图像进行表征显示其结构完整、分散均匀.本工作为后续的单分子磁共振技术在单分子生物物理领域的应用推广奠定了样品制备的基础.
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| 关 键 词： | 金刚石 DNA折纸 单分子磁共振 样品制备 |
| 收稿时间： | 2019/9/5 0:00:00 |
| 修稿时间： | 2019/10/18 0:00:00 |
Preparation of DNA Origami for Ordered Immobilization of Single Molecules on Diamond Surface |
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| WEI Yi-Cheng,YU Pei,CHEN Ming,SUN Zi-Ting,ZENG Yan,LOU Ji-Zhong,CHEN San-You,SHI Fa-Zhan and DU Jiang-Feng.Preparation of DNA Origami for Ordered Immobilization of Single Molecules on Diamond Surface[J].Progress In Biochemistry and Biophysics,2020,47(1):53-60. |
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| Authors: | WEI Yi-Cheng YU Pei CHEN Ming SUN Zi-Ting ZENG Yan LOU Ji-Zhong CHEN San-You SHI Fa-Zhan and DU Jiang-Feng |
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| Institution: | 1)Hefei National Laboratory for Physical Sciences at the Microscale, University of Science and Technology of China, Hefei 230026, China;2)CAS Key Laboratory of Microscale Magnetic Resonance and Department of Modern Physics, University of Science and Technology of China, Hefei 230026, China,1)Hefei National Laboratory for Physical Sciences at the Microscale, University of Science and Technology of China, Hefei 230026, China;2)CAS Key Laboratory of Microscale Magnetic Resonance and Department of Modern Physics, University of Science and Technology of China, Hefei 230026, China,1)Hefei National Laboratory for Physical Sciences at the Microscale, University of Science and Technology of China, Hefei 230026, China;2)CAS Key Laboratory of Microscale Magnetic Resonance and Department of Modern Physics, University of Science and Technology of China, Hefei 230026, China,1)Hefei National Laboratory for Physical Sciences at the Microscale, University of Science and Technology of China, Hefei 230026, China;2)CAS Key Laboratory of Microscale Magnetic Resonance and Department of Modern Physics, University of Science and Technology of China, Hefei 230026, China,3)Key Laboratory of RNA Biology, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China,3)Key Laboratory of RNA Biology, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China,1)Hefei National Laboratory for Physical Sciences at the Microscale, University of Science and Technology of China, Hefei 230026, China;2)CAS Key Laboratory of Microscale Magnetic Resonance and Department of Modern Physics, University of Science and Technology of China, Hefei 230026, China,1)Hefei National Laboratory for Physical Sciences at the Microscale, University of Science and Technology of China, Hefei 230026, China;2)CAS Key Laboratory of Microscale Magnetic Resonance and Department of Modern Physics, University of Science and Technology of China, Hefei 230026, China,1)Hefei National Laboratory for Physical Sciences at the Microscale, University of Science and Technology of China, Hefei 230026, China;2)CAS Key Laboratory of Microscale Magnetic Resonance and Department of Modern Physics, University of Science and Technology of China, Hefei 230026, China |
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| Abstract: | |
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| Keywords: | diamond DNA origami single-molecule magnetic resonance sample preparation |
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