Substrate and product inhibition kinetics in succinic acid production by Actinobacillus succinogenes |
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Authors: | Sze Ki Carol Lin Chenyu Du Apostolis Koutinas Ruohang Wang Colin Webb |
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Institution: | aSatake Centre for Grain Process Engineering, School of Chemical Engineering and Analytical Science, the University of Manchester, P.O. Box 88, Manchester M60 1QD, UK |
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Abstract: | The inhibition of substrate and products on the growth of Actinobacillus succinogenes in fermentation using glucose as the major carbon source was studied. A. succinogenes tolerated up to 143 g/L glucose and cell growth was completely inhibited with glucose concentration over 158 g/L. Significant decrease in succinic acid yield and prolonged lag phase were observed with glucose concentration above 100 g/L. Among the end-products investigated, formate was found to have the most inhibitory effect on succinic acid fermentation. The critical concentrations of acetate, ethanol, formate, pyruvate and succinate were 46, 42, 16, 74, 104 g/L, respectively. A growth kinetic model considering both substrate and product inhibition is proposed, which adequately simulates batch fermentation kinetics using both semi-defined and wheat-derived media. The model accurately describes the inhibitory kinetics caused by both externally added chemicals and the same chemicals produced during fermentation. This paper provides key insights into the improvement of succinic acid production and the modelling of inhibition kinetics. |
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Keywords: | Fermentation Product inhibition Substrate inhibition Growth kinetic Succinic acid Actinobacillus succinogenes |
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