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水中病原微生物分子检测技术研究进展
引用本文:赵仲麟,李淑英,李燕,苏同福,金显春,袁超,胡章立.水中病原微生物分子检测技术研究进展[J].生物技术通报,2012(1):41-45.
作者姓名:赵仲麟  李淑英  李燕  苏同福  金显春  袁超  胡章立
作者单位:1. 河南农业大学理学院,郑州,450002
2. 中国农业科学院农产品加工研究所,北京,100193
3. 郑州轻工业学院食品与生物工程学院,郑州,450002
4. 深圳大学生命科学学院,深圳,518060
基金项目:国家水体污染控制与治理科技重大专项资助项目
摘    要:基于PCR方法的多种分子检测技术已广泛的应用于水体病原微生物的检测中。而以DNA芯片为代表的微型化、快速化手段将是未来检测技术的发展方向,可实现对病原微生物实时和快速的检测。新检测技术的发展有利于建立水体污染早期预警机制,同时,可靠的病原微生物检测方法可降低有害微生物对人类健康的影响。对水体病原微生物分子检测方法及其在水污染相关疾病风险控制中所扮演的重要角色进行阐述。

关 键 词:分子检测  水中病原微生物  DNA芯片  PCR

Research Advances in Molecular Detection Techniques of Waterborne Pathogens
Zhao Zhonglin , Li Shuying , Li Yan , Su Tongfu , Jin Xianchun , Yuan Chao , Hu Zhangli.Research Advances in Molecular Detection Techniques of Waterborne Pathogens[J].Biotechnology Bulletin,2012(1):41-45.
Authors:Zhao Zhonglin  Li Shuying  Li Yan  Su Tongfu  Jin Xianchun  Yuan Chao  Hu Zhangli
Institution:1College of Sciences,Henan Agricultural University,Zhengzhou 450002;2Institute of Agro-products Processing Science and Technology,Chinese Academy of Agricultural Sciences,Beijing 100193;3School of Food and Biological Engineering,Zhengzhou University of Light Industry,Zhengzhou 450002;4College of Life Sciences,Shenzhen University,Shenzhen 518060)
Abstract:Numbers of molecular techniques,such as PCR method,were developed for detection of waterborne pathogens.DNA microarray offered a novel mean to enhance the detection of waterborne pathogens.The future developments in quick detection and miniaturization technologies would provide real-time monitoring for rapid assessment of microbial pathogens.New techniques could aid development of strong early warning systems and allow effective remediation of impacts from harmful microorganisms.This paper summarired development of new monitoring techniques and discussed important role in risk management of water related diseases.
Keywords:Molecular detection Waterborne pathogen DNA microarray PCR
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