| 一株絮凝菌的鉴定、絮凝基因定位及其絮凝剂成分分析 |
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| 引用本文： | 陈俊,詹志钢,陈哲,王涛,杨之帆.一株絮凝菌的鉴定、絮凝基因定位及其絮凝剂成分分析[J].生物技术通报,2012(4):122-126. |
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| 作者姓名： | 陈俊 詹志钢 陈哲 王涛 杨之帆 |
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| 作者单位： | 1. 武汉科技大学化学工程与技术学院,武汉,430081
2. 湖北大学生命科学学院,武汉,430062 |
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| 摘 要： | 从武汉市综合废水处理的活性污泥中筛选得到一株絮凝剂产生菌株MBF03,其絮凝率达到90％以上,絮凝效果稳定.通过16S rDNA鉴定,该菌为泛菌属,扫描电镜结果显示为杆状.通过质粒转化与质粒消除试验,初步证实了MBF03菌的絮凝基因位于染色体上.提取该菌所产的絮凝剂,进行紫外、红外分析,结果表明,该絮凝剂主要成分是胞外多糖类物质,不含蛋白质和核酸.
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| 关 键 词： | 絮凝菌 16S rDNA鉴定 絮凝基因 絮凝剂 |
Identification, Flocculation Gene Mapping and Flocculant Composition of a Flocculation Bacteria |
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| Chen Jun
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Zhan Zhigang
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Chen Zhe
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Wang Tao
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Yang Zhifan.Identification, Flocculation Gene Mapping and Flocculant Composition of a Flocculation Bacteria[J].Biotechnology Bulletin,2012(4):122-126. |
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| Authors: | Chen Jun Zhan Zhigang Chen Zhe Wang Tao Yang Zhifan |
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| Institution: | 1 College of Chemical Engineering and Technology,Wuhan University of Science and Technology,Wuhan 430081;2 College of Life Sciences,Hubei University,Wuhan 430062) |
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| Abstract: | One flocculant-producing strain named MBF03 was isolated from the activated sludge of Wuhan integrated wastewater treatment factory,of which flocculation rate reached 90%.Sequencing of the 16S rDNA gene revealed identification as Pantoea sp..Scanning electron microscopy showed that MBF03 was rod-shape.With plasmid transformation and plasmid elimination experiments,we primary confirmed that the flocculation gene of MBF03 bacteria was located on chromosome DNA rather than a plasmid.Subsequent ultraviolet and infrared analysis on MBF03 flocculants revealed the main component of the extracellular flocculants was polysaccharide rather than protein and/or nucleic acid. |
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| Keywords: | Flocculation bacteria 16S rDNA identification Flocculation gene Flocculant |
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