| 人骨形态发生蛋白7在中国仓鼠卵巢细胞中的表达研究 |
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| 引用本文： | 杨允,邓春梅,韩明,黄艺娜,黄义德.人骨形态发生蛋白7在中国仓鼠卵巢细胞中的表达研究[J].生物技术,2012,22(2):23-26. |
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| 作者姓名： | 杨允 邓春梅 韩明 黄艺娜 黄义德 |
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| 作者单位： | 福建师范大学生命科学学院,福建福州350108;福建省发育与神经生物学重点实验室,福建福州350108 |
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| 摘 要： | 目的:构建重组人骨形态发生蛋白-7(rhBMP7)表达质粒,并研究其在中国仓鼠卵巢细胞中的表达。方法:将hBMP7重组表达质粒电转到中国仓鼠卵巢细胞(CHO)中,并用DOT-BLOT和ELISA方法分析检测rhBMP7在重组CHO细胞中的表达。结果:hBMP7 cDNA整合到CHO细胞基因组中并被转录。点杂交和ELISA检测证实rhBMP7在CHO细胞中得到表达。结论:hBMP7成功在CHO表达系统中得到表达。
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| 关 键 词： | 人骨形态发生蛋白-7 中国仓鼠卵巢细胞 重组表达 |
Expression of Human Bone Morphogenetic Protein 7 in Chinese Hamster Ovary Cells |
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| YANG Yun
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DENG Chun-mei
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HAN Ming
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HUANG Yi-na
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HUANG Yi-de.Expression of Human Bone Morphogenetic Protein 7 in Chinese Hamster Ovary Cells[J].Biotechnology,2012,22(2):23-26. |
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| Authors: | YANG Yun DENG Chun-mei HAN Ming HUANG Yi-na HUANG Yi-de |
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| Institution: | 1,2(1.College of Life Sciences,Fujian Normal university,Fuzhou 350108,China; 2.Fujian Key Laboratory of Developmental and NeuroBiology,Fuzhou 350108,China) |
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| Abstract: | Objective:Construction of the recombinant human bone morphogenetic protein 7(rhBMP7) expression vector and expression of rhBMP7 in Chinese Hamster Ovary(CHO) cells.Method:The expression vectors containing hBMP7 cDNA was transformed into CHO cells by electroporation and expression levels of rhBMP7 was analyzed by Dot blot and ELISA.Result:hBMP7 cDNA was integrated into genome of CHO cells and was transcribed in recombinant cells.Expression of rhBMP7 was con-firmed by Dot blot and ELISA.Conclusion:hBMP7 was successfully expressed in CHO expression system. |
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| Keywords: | human bone morphogenetic protein7(hBMP7) CHO cells recombinant expression |
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