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| 烟草蛋白酶抑制剂基因的电子克隆及生物信息学分析 | |
| 引用本文： | 林世锋,王仁刚,任学良. 烟草蛋白酶抑制剂基因的电子克隆及生物信息学分析[J]. 生物技术通报, 2012, 0(4): 80-86 |
| 作者姓名： | 林世锋 王仁刚 任学良 |
| 作者单位： | 贵州省烟草科学研究所,贵阳,550081 |
| 基金项目： | 贵州省烟草专卖局(公司)科技专项，贵州省科学技术基金项目 |
| 摘 要： | 蛋白酶抑制剂可以增强植物对病虫害的抵抗能力,为了深入研究蛋白酶抑制剂在烟草中的作用机制,利用生物信息学的方法,成功获得了烟草品种K326中的4种蛋白酶抑制剂cDNA序列(NtPI-1、NtPI-2、NtPI-3和NtPI-4)并对其进行了序列分析.它们编码的氨基酸序列都具有典型的马铃薯蛋白酶抑制剂Ⅰ家族功能结构域,属于马铃薯蛋白酶抑制剂Ⅰ家族成员.序列分析表明,4种蛋白酶抑制剂基因cDNA序列均具有完整的开放读码框,依次编码128、95、94和72个氨基酸残基,它们的氨基酸序列一致性在31％-38％之间.系统树分析表明,烟草品种K326中的4种蛋白酶抑制剂分散在进化树的不同位置,形成不同亚群. |
| 关 键 词： | 烟草 蛋白酶抑制剂 电子克隆 生物信息学 |
In Silicon Cloning and Bioinformatic Analysis of Proteinase Inhibitor Genes in Tobacco |
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| Lin Shifeng , Wang Rengang , Ren Xueliang. In Silicon Cloning and Bioinformatic Analysis of Proteinase Inhibitor Genes in Tobacco[J]. Biotechnology Bulletin, 2012, 0(4): 80-86 | |
| Authors: | Lin Shifeng Wang Rengang Ren Xueliang |
| Affiliation: | Lin Shifeng Wang Rengang Ren Xueliang(Guizhou Tobacco Research Institute,Guiyang 550081) |
| Abstract: | Proteinase inhibitors(PIs)have been found to induce plant defense response.For further studying the physiological functions and mechanisms of PIs in tobacco(Nicotiana tabacum),we got four distinct PI cDNAs(NtPI-1,NtPI-2,NtPI-3 and NtPI-4)successfully from tobacco cultivar K326 using in silicon cloning technique,and then they were analyzed by the method of bioinformatics.The results showed that four of them had a complete open reading frame and encoded 128,95,94 and 72 amino acid residues,respectively,including the conserved domains of the potato protease inhibitor I family.The identity of their deduced amino acid sequences varied from 31% to 38%,and phylogenetic analysis indicated they belonged to different subgroups and scattered across the evolutionary tree. |
| Keywords: | Nicotiana tabacum Proteinase inhibitor In siliconcloning Bioinformatics |
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