| C2C12成肌细胞诱导肌性分化过程中miR-101a的表达 |
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| 引用本文： | 李粉,王丽,郑艳艳,陈华群. C2C12成肌细胞诱导肌性分化过程中miR-101a的表达[J]. 生物技术通报, 2012, 0(1): 116-119 |
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| 作者姓名： | 李粉 王丽 郑艳艳 陈华群 |
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| 作者单位： | 南京师范大学生命科学学院江苏省分子医学生物技术重点实验室,南京,210046 |
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| 基金项目： | "973"计划项目 |
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| 摘 要： | 以C2C12成肌细胞为模型,在分化培养基中诱导C2C12建立体外肌性细胞分化模型.以poly (A)3′-端加尾和实时定量PCR方法研究miR-101a在C2C12细胞分化过程中的表达情况.结果发现,在细胞转入分化培养基进行肌性分化的1-5 d中,miR-101a的表达量逐渐增加,提示miR-101a可能在肌肉发生中发挥调控作用.
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| 关 键 词： | C2C12C成肌细胞 实时定量PCR miR-101a 肌分化 |
The Expression of miR-101a in C2C12 Myoblast During Myogenic Differentiation |
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| Li Fen , Wang Li , Zheng Yanyan , Chen Huaqun. The Expression of miR-101a in C2C12 Myoblast During Myogenic Differentiation[J]. Biotechnology Bulletin, 2012, 0(1): 116-119 |
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| Authors: | Li Fen Wang Li Zheng Yanyan Chen Huaqun |
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| Affiliation: | Li Fen Wang Li Zheng Yanyan Chen Huaqun (Jiangsu Key Laboratory for Molecular and Medical Biotechnodogy,College of Life Sciences,Nanjng Normal University,Nanjing 210046) |
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| Abstract: | In order to establish muscle differentiation model,the C2C12 myoblast cells were incubated in differentiation medium(DM) and induced to myogenic differentiation.The expression of mature miR-101a was examined by poly(A) tail addition at 3’-end and Real-time PCR method.The results showed that the level of mature miR-101a was gradually increased during the induction time from 1 day to 5 days after the cells changed to DM,suggesting that miR-101a may play regulatory roles in myogenesis. |
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| Keywords: | C2C12 myoblast Real-time PCR miR-101a Myogenic differentiation |
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