| 鹿茸表皮生长因子 |
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| 引用本文： | 江润祥,高锦明,叶大同,曹世华.鹿茸表皮生长因子[J].动物学报,1987(4). |
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| 作者姓名： | 江润祥 高锦明 叶大同 曹世华 |
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| 作者单位： | 香港中文大学生物化学系及解剖系
(江润祥，高锦明，叶大同)，香港中文大学生物化学系及解剖系(曹世华) |
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| 摘 要： | 作者等通过应用一凝肢柱或DEAE凝胶柱和固定金属离子亲和凝腔柱(Lmmobilised Metal Affinity chromatography Column,IMAC)的组合,成功地从梅花鹿的鹿茸表皮层中分离出表皮生长因子(Epidermal Growth Factor, EGF)。此半纯化的EGF能刺激小鼠Balb/c 3T3的静态成纤维细胞对~3H-胸嘧啶的渗入,渗入量随EGF剂量的增加而升高。在鹿茸表皮层的组织中亦同时含有大量的EGF受体,而骨轴组织不舍有EGF,只含少量受体。
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EPIDERMAL GROWTH FACTOR OF THE CERVINE VELVET ANTLER |
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| KONG YUNCHEUNO KO KAMMING YIP TAITUNC TSAO SAIWAH.EPIDERMAL GROWTH FACTOR OF THE CERVINE VELVET ANTLER[J].Acta Zoologica Sinica,1987(4). |
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| Authors: | KONG YUNCHEUNO KO KAMMING YIP TAITUNC TSAO SAIWAH |
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| Abstract: | Epidermal growth factor (EGF) was isolated from the velvet antler of Chinsse spotted deer (Cervus nippon) by a combination of DEAE-Sephacel or Sephadex G-50 and Immobilised Metal Affinity chromatography (IMAC) columns in succession. The semi-purified EGF could induce the incorporation of 3H-thymidine in quiescent Balb/C 3T3 mouse fibroblasts culture in a dose-dependant manner. EGF receptor was also detected in the velvet tissues; but the antler shaft contained no EGF and few EGF receptors. |
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| Keywords: | Epidermal growth factor (EGF) Deer antler Immobilised metal affinity chromatography (IMAC) |
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