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Dopamine receptors on photoreceptor membranes couple to a GTP-binding protein which is sensitive to both pertussis and cholera toxin
Authors:M R Brann  C L Jelsema
Affiliation:1. Department of Chemistry, Washington State University, Pullman, WA, 99163, USA;2. Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, The British University in Egypt, El-Sherouk City, 11837, Egypt;3. Analytical Chemistry Department, Faculty of Pharmacy, Cairo University, Kasr El Aini, Cairo, 11562, Egypt;4. Analytical Chemistry Department, Faculty of Pharmacy, Helwan University, Ein Helwan, Cairo, Egypt;1. Department of Microbiology and Plant Pathology, University of California, Riverside, Riverside, CA, USA;2. Department of Biochemistry, University of California, Riverside, Riverside, CA, USA;3. Graduate Program in Genetics, Genomics, and Bioinformatics, University of California, Riverside, Riverside, CA, USA;4. Division of Biomedical Sciences, School of Medicine, University of California, Riverside, Riverside, CA, USA;1. Epicentre, Paris, France;2. London School of Hygiene & Tropical Medicine, London, UK;3. World Health Organization, Sana''a, Yemen;4. Health Authorities, Yemen;5. Central Public Health Laboratory, Sana''a, Yemen;6. World Health Organization, Cairo, Egypt;7. Médecins Sans Frontières, Dubai, United Arab Emirates;8. Médecins Sans Frontières, Barcelona, Spain;9. Médecins Sans Frontières, Geneva, Switzerland;10. Médecins Sans Frontières, Amsterdam, Netherlands;11. Médecins Sans Frontières, Sana''a, Yemen;12. World Health Organization, Geneva, Switzerland;13. Johns Hopkins School of Public Health, Baltimore, MD, USA;14. UNICEF, Sana''a, Yemen;15. National Reference Center for Vibrios and Cholera, Institut Pasteur, Paris, France;1. Behavior and Basal Ganglia Research Unit (EA 4712), University of Rennes 1, Rennes, France;2. Institut des Neurosciences Cliniques de Rennes (INCR), Rennes, France;3. Movement Disorders Unit, Neurology Department, Pontchaillou University Hospital, Rennes, France
Abstract:Dopamine receptors with a pharmacological profile similar to D2 receptors are coenriched with rhodopsin in preparations of bovine retinal membranes. A high density of these receptors are present on photoreceptor membranes. The affinity of the agonist apomorphine for these receptors is decreased by the guanine nucleotides GTP and GppNHp. Treatment of photoreceptor membranes with pertussis or cholera toxin also decreased the affinity of apomorphine and eliminated the effect of GTP.
Keywords:
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