(1) Department of Plant Biology, The Genetics Center, The Swedish University of Agricultural Sciences, P.O. Box, 7080, SE-, 75007, Uppsala;(2) Development Corporation Limited, P.O. Box, Genesis Research, 50, Auckland, New Zealand
Abstract:
The construction of four plasmids optimized for transgenic studies in barley and other monocot plants is presented. All vectors contain the promoter and first intron of the rice actin 1 (Act-1) promoter to drive expression of the coding sequence of choice. Two of the vectors utilize the gene for green fluorescent protein (GFP) from Aequorea victoria as a screenable marker.