| 透明颤菌血红蛋白基因在解淀粉芽孢杆菌中的表达及其影响 |
| |
| 引用本文： | 刘清术,夏立秋,罗玉双,李敬业,张允雷,丁学知,胡胜标,余子全,孙运军. 透明颤菌血红蛋白基因在解淀粉芽孢杆菌中的表达及其影响[J]. 激光生物学报, 2010, 19(6): 790-797. DOI: 10.3969/j.issn.1007-7146.2010.06.013 |
| |
| 作者姓名： | 刘清术 夏立秋 罗玉双 李敬业 张允雷 丁学知 胡胜标 余子全 孙运军 |
| |
| 作者单位： | [1]湖南师范大学生命科学学院微生物分子生物学湖南省重点实验室,湖南长沙410081 [2]湖南省微生物研究所,湖南长沙410009 |
| |
| 基金项目： | 国家自然科学基金项目，国家"863 计划"项目 |
| |
| 摘 要： | 将强启动子P43与透明颤菌血红蛋白基因（vgb）通过重叠延伸PCR进行融合,克隆到芽孢杆菌整合表达载体pDG1730中,重组表达载体pDG-P43vgb转化促生防病解淀粉芽孢杆菌FZB42,Wsetern-Blot和CO差光谱分析表明重组菌株FZB42-VHb表达了有活性的VHb蛋白,VHb的表达对重组菌株菌体的生长及抗菌脂肽的产生都有促进作用.在相同培养条件下,重组菌最大菌体密度比原始菌株提高了14.49 %,抗菌脂肽fengycin的产量提高了1.74倍,抗菌脂肽surfactin的产量提高了3.14倍.
|
| 关 键 词： | 透明颤菌血红蛋白基因 解淀粉芽孢杆菌 抗菌脂肽 |
Expression of Vitreoscilla Hemoglobin Gene in Bacillus amyloliquefaciens and Its Effect |
| |
| LIU Qing-shu,XIA Li-qiu,LUO Yu-shuang,LI Jing-ye,ZHANG Yun-lei,DING Xue-zhi,HU Sheng-biao,YU Zi-quan,SUN Yun-jun. Expression of Vitreoscilla Hemoglobin Gene in Bacillus amyloliquefaciens and Its Effect[J]. ACTA Laser Biology Sinica, 2010, 19(6): 790-797. DOI: 10.3969/j.issn.1007-7146.2010.06.013 |
| |
| Authors: | LIU Qing-shu XIA Li-qiu LUO Yu-shuang LI Jing-ye ZHANG Yun-lei DING Xue-zhi HU Sheng-biao YU Zi-quan SUN Yun-jun |
| |
| Affiliation: | 1. Key Laboratory of Microbial Molecular Biology of Hunan Province, Life Science College of Hunan Normal University, Changsha 410081, Hunan, China; 2. Hunan Institute of Microbiology, Changsha 410009, Hunan, China) |
| |
| Abstract: | The Vitreoscilla hemoglobin （VHb） gene （vgb） was placed under the control of the P43 promoter by overlapping extension PCR. The vgb expression cassette was integrated into the chromosome of Bacillus amyloliquefaciens FZB42 using integrative vector pDG1730. The production of VHb confirmed by Western-Blot and CO-difference spectra analysis, promoted the growth of the strain and improved the yield of the antimicrobial lipopeptide. The maximum viable cell counts of the FZB42-VHb strain were 1.14 fold of those of the host strain. Under the same conditions,the yeild of the antimicrobial lipopeptide fengycin and surfactin were 1.74-,3.14-fold of those of the host strain,respectively. |
| |
| Keywords: | Vitreoscilla hemoglobin （VHb） gene Bacillus amyloliquefaciens antimicrobial lipopeptide |
| 本文献已被 维普 万方数据 等数据库收录！ |
|
