Changes in cardiac Na+/K+-ATPase expression and activity in female rats fed a high-fat diet |
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| Authors: | Aleksandra Jovanovic Milan Obradovic Emina Sudar Milovanovic Alan J. Stewart Samantha J. Pitt Dragan Alavantic Ema Aleksic Esma R. Isenovic |
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| Affiliation: | 1.Laboratory of Radiobiology and Molecular Genetics, Institute of Nuclear Sciences Vinca,University of Belgrade,Belgrade,Serbia;2.School of Medicine,University of St Andrews,St Andrews,UK;3.Faculty of Dentistry in Pancevo,University Business Academy,Belgrade,Serbia |
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| Abstract: | The aim of this study was to investigate whether the presence of endogenous estradiol alters the effects of a high-fat (HF) diet on activity/expression of the cardiac Na+/K+-ATPase, via PI3K/IRS and RhoA/ROCK signalling cascades in female rats. For this study, female Wistar rats (8 weeks old, 150–200 g) were fed a standard diet or a HF diet (balanced diet for laboratory rats enriched with 42% fat) for 10 weeks. The results show that rats fed a HF diet exhibited a decrease in phosphorylation of the α1 subunit of Na+/K+-ATPase by 30% (p < 0.05), expression of total α1 subunit of Na+/K+-ATPase by 31% (p < 0.05), and association of IRS1 with p85 subunit of PI3K by 42% (p < 0.05), while the levels of cardiac RhoA and ROCK2 were significantly increased by 84% (p < 0.01) and 62% (p < 0.05), respectively. Our results suggest that a HF diet alters cardiac Na+/K+-ATPase expression via molecular mechanisms involving RhoA/ROCK and IRS-1/PI3K signalling in female rats. |
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