中药灵芝降羊毛甾烷三萜类成分研究

研究灵芝Ganoderma lingzhi子实体的化学成分。采用正相硅胶、ODS、Sephadex LH-20凝胶柱色谱和prep-HPLC等方法分离与纯化,运用NMR、MS等波谱技术鉴定化合物结构。从灵芝95%乙醇提取物中分离得到5个降羊毛甾烷三萜类化合物,分别是ethyl 20(21)-dehydrolucidenate A(1)、lingzhi-20(21)-en-24-oic acid A(2)、20(21)-dehydrolucidenic acid A(3)、赤芝酮A(4)、lucidadone H(5)。化合物1和2是两个新的降羊毛甾烷三萜化合物;化合物3~5为首次从该属真菌中分离得到。化合物1和2在白血病(HL-60)、肺癌(A549)、肝癌(SMMC-7721)、乳腺癌(MCF-7)、结肠癌(SW480)五种不同的癌细胞株上进行细胞毒活性筛选,结果显示化合物1~5在40μM时无明显的细胞毒活性。

Study on lanostane-type nortriterpenoids from Ganoderma lingzhi

To study the chemical constituents from the fruiting bodies of Ganoderma lingzhi,all compounds were isolated and purified by various column chromatographies,including silica gel,ODS,Sephadex LH-20 and prep-HPLC,and their structures were identified by NMR,MS and other spectroscopic techniques.Five lanostane-type nortriterpenoids were isolated from 95%ethanol extract of G.lingzhi,which were identified as ethyl 20(21)-dehydrolucidenate A(1),lingzhi-20(21)-en-24-oic acid A(2),20(21)-dehydrolucidenic acid A(3),lucidone A(4),and lucidadone H(5).Compounds 1 and 2 have not been described before,and compounds 3-5 are isolated from this fungus for the first time.Cytotoxic effects of compounds 1-5 were investigated against five regular cancer cell lines,and the results showed that none of them showed significant activity.