羊角拗茎的化学成分及其抗炎活性研究

探讨羊角拗茎的化学成分及其抗炎活性,采用硅胶、Sephadex LH-20等色谱分离技术分离纯化羊角拗茎乙醇提取物的乙酸乙酯萃取物中的化合物,通过质谱和核磁共振等波谱技术对其结构进行鉴定,确定出了13个化合物,包括常春藤皂苷元(1)、(-)-loliolide(2)、(3 S,5 R,6 S,7 E)-3,5,6-trihydroxy-7-megastigmen-9-one(3)、(3 R,6 R,7 E)-3-hydroxy-4,7-megastigmadien-9-one(4)、松脂素(5)、表松脂素(6)、4,4′-dihydroxy-3,3′-dimethoxybenzophenone(7)、6-羟基柚皮素(8)、东莨菪素(9)、6-羟基-7,8-二甲氧基香豆素(10)、吲唑(11)、香草酸(12)、对羟基苯甲酸(13)。其中化合物1~4和6~13为首次从羊角拗中分离得到。采用LPS诱导小鼠单核巨噬细胞RAW 264.7体外细胞炎症模型测试结果表明,化合物5能显著抑制NO的产生,IC₅₀值为18.09±1.09μM。

Study on chemical constituents and their anti-inflammatory activity from stems of Strophanthus divaricatus

To study the chemical constituents and their anti-inflammatory activity from the stems of Strophanthus divaricatus,thirteen compounds were isolated from its ethyl acetate fraction of ethanol extract by using chromatographic techniques including silica gel,Sephadex LH-20 etc.Their structures were elucidated on the basis of spectroscopic data analysis as hederagenin(1),(-)-loliolide(2),(3 S,5 R,6 S,7 E)-3,5,6-trihydroxy-7-megastigmen-9-one(3),(3 R,6 R,7 E)-3-hydroxy-4,7-megastigmadien-9-one(4),(+)-pinoresinol(5),epipinoresinol(6),4,4′-dihydroxy-3,3′-dimethoxybenzophenone(7),6-hydroxynaringenin(8),scosolrtin(9),6,7-dimethylesculetin(10),indazole(11),vanillic acid(12),and p-hydroxybenzoic acid(13).Compounds 1~4 and 6~13 were identified from this plant for the first time.Compound 5 showed a significant inhibition on the lipopolysaccharide(LPS)-induced nitric oxide(NO)production in RAW 264.7 cells with IC₅₀ value of 18.09±1.09μM.