| 树状多节孢发酵生产紫杉醇工艺条件的初步研究* |
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| 引用本文： | 刘晓兰,周东坡,孙剑秋,平文祥,于寒颖,张鹏,解玉红,凌洪博.树状多节孢发酵生产紫杉醇工艺条件的初步研究*[J].菌物学报,2002,21(2):246-251. |
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| 作者姓名： | 刘晓兰 周东坡 孙剑秋 平文祥 于寒颖 张鹏 解玉红 凌洪博 |
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| 作者单位： | 1. 齐齐哈尔大学生命科学与工程学院,齐齐哈尔,161006
2. 黑龙江大学生命科学学院,哈尔滨,150080 |
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| 基金项目： | 黑龙江省自然科学基金资助项目(93-C-11)，黑龙江省“九五”重大攻关项目(G98C20-16-1) |
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| 摘 要： | 研究了树状多节孢HQD33内生真菌融合子TPF-1摇瓶发酵工艺条件,进行了2.8L和10L通用式机械搅拌罐的发酵试验。结果表明,HQD33适宜发酵工艺条件是:发酵时间16～18d,培养基中蔗糖、苯丙氨酸、醋酸钠、酪氨酸、2,4-二氯苯氧乙酸和亚油酸的加量分别为10g/L、1mg/L、1.5g/L、15mg/L、5mg/L和15mg/L,摇瓶装量为150ml/500ml,在此条件下摇瓶发酵液中紫杉醇平均含量为448.52 g/L; 2.8L和10L罐发酵液中紫杉醇含量达406.95和395.12g/L(平均值)。
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| 关 键 词： | 树状多节孢 紫杉醇 发酵 |
| 文章编号： | 1007-3515(2002)02-0246-0251 |
| 修稿时间： | 2001年12月24 |
A STUDY ON FERMENTATION PROCESS FOR PRODUCTION OF TAXOL BY NODULISPORIUM SYLVIFORME |
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| Authors: | LIU Xiao-Lan ZHOU Dong-Po SUN Jian-Qiu PING Wen-Xiang YU Han-Ying ZHANG Peng XIE Yu-Hong LING Hong-Bo |
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| Institution: | LIU Xiao-Lan1 ZHOU Dong-Po1 SUN Jian-Qiu1 PING Wen-Xiang2 YU Han-Ying1 ZHANG Peng1 XIE Yu-Hong1 LING Hong-Bo1 |
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| Abstract: | A flask fermentation process by fusant TFP-1 from Nodulisporium sylviforme HQD33 was studied, and fermentation tests in batch stirred tank reactors of 2.8 L and 10L were conducted. Suitable fermentation time was 16~18d, suitable contents of some components in medium were sucrose 10g/L, Phe 1mg/L, sodium acetate 1.5g/L, Tyr 15mg/L, 2,4-D 5mg/L, linoleic acid 15mg/L respectively, and medium volume in 500ml flask was 150ml. Taxol produced in optimized flask culture reached 448.52靏/L, and average values of Taxol produced in 2.8L and 10L BSTR reached 406.95 and 395.12靏/L respectively. |
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| Keywords: | Nodulisporium sylviforme taxol fermentation |
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