异丙酚和氯胺酮对大鼠离体缺血再灌注损伤心肌脂质过氧化的影响

目的：比较异丙酚和氯胺酮对大鼠离体缺血再灌注损伤心肌脂质过氧化的影响。方法：成年Wistar大鼠18只，雌雄不拘。体重240-300g，随机分为3组（T1=6）：心肌缺血再灌注损伤组（I／R组），异丙酚组（P组），氯胺酮组（K组）。采用Langendorff灌装置建立离体心脏缺血再灌注模型，将心脏连接至Langendorff逆灌装置，3组均以K-H液平衡灌注10min后，再分别以K．H液、含30μmol/L。异丙酚的K-H液、含10μmol-L-1氯胺酮的K-H液灌注10min，然后全心停灌25min，再分别以停灌前相同的灌注液恢复灌注30min。留取冠脉流出液测定总LDH活性；灌注末取左室心肌组织置于2．5％的戊二醛固定，观察心肌的超微结构；心尖部心肌组织留待检测8-异前列腺素和SOD活性。结果：与I／R组比较，P组8-异前列腺素含量降低，SOD活性升高，LDH活性降低（P〈0．05）；K组8-异前列腺素含量，SOD及LDH活性均无统计学意义（P〉0．05）；与P组比较，K组8-异前列腺素含量升高，SOD及LDH活性降低（P〈0．05）；P组心肌超微结构损伤较m组和K组也明显改善。结论：异丙酚可显著减轻心肌缺血再灌注损伤大鼠的脂质过氧化和心肌缺血再灌注损伤，而氯胺酮没有抗心肌缺血再灌注损伤心肌脂质过氧化的作用。

Effects of Propofol and Ketamine on Lipid Peroxidation in Isolated Rat Hearts after Myocardial Ischemia-Reperfusion

Objective： To compare the effects ofpropofol and ketamine on lipid peroxidation in isolated rat hearts after myocardial ischemia-reperfusion. Methods： 18 adult Wistar rats weighing 240-300 g were randomly divided into 3 groups（n=6）： ischemia-reperfusion group（I/R group）, propofol group（group P）, ketamine group（group K）. The hearts of 18 wistar rats were removed, mounted on a langen- dorffapparatus, perfused with Krebs-Henseleit （K-H） solution for 10 min, and then perfused with K-H solution contained no anesthetics, 30 propofol, 10 g, mol.L-1 ketarnine for 10 min respectively, followed by 25 min global ischemia and 30 min reperfusion. At the end of the reperfusion, the reperfusiori K-H solution was sampled for lactate dehydrogenase（LDH） measurement. The tissue samples from apex of hearts were obtained to measure the level of 8-isoprostane, SOD activity. Results： Compared with I/R group and group K, 8-iso- prostane and LDH levels were decreased, while the SOD activity was increased in group P （P〈0.05）. Compared with I/R group, 8-iso- prostane and LDH levels were increased in group K, while the SOD activity was decreased （P〉0.05）. The microscopic examination showed that propofol can alleviate the myocardial UR injury, while ketamine cannot. Conclusion： Propofol could attenuate lipid peroxida- tion in isolated rat hearts after myocardial ischemia-reperfusion, and had protective effects against myocardial I/R injury, while ketamine did not have these effects.