Trafficking of the plant potassium inward rectifier KAT1 in guard cell protoplasts of Vicia faba |
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Authors: | Hurst Annette C Meckel Tobias Tayefeh Sascha Thiel Gerhard Homann Ulrike |
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Institution: | Institute of Botany, Technical University of Darmstadt, 64287 Darmstadt, Germany. |
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Abstract: | Trafficking of K+ inward (Kin+) rectifying channels was analyzed in guard cells of Vicia faba transfected with the Kin+ rectifier from Arabidopsis thaliana KAT1 fused to the green fluorescent protein (GFP). Confocal images and whole-cell patch-clamp measurements confirmed the incorporation of active KAT1 channels into the plasma membrane of transfected guard cell protoplasts. The Kin+ rectifier current density of the plasma membrane was much larger in transfected protoplasts than in wild-type (wt) protoplasts. This shows a coupling between K+ channel synthesis and incorporation of the channel into the plasma membrane. Pressure-driven increase and decrease in surface area led to the incorporation and removal of vesicular membrane carrying active Kin+ rectifier in wt and transfected protoplasts. These vesicular membranes revealed a higher channel density than the plasma membrane, suggesting that Kin+ rectifier remains in clusters during trafficking to and from the plasma membrane. The observed results can be explained by a model illustrating that vesicles of a pre-plasma membrane pool carry K+ channels preferentially in clusters during constitutive and pressure-driven exo- and endocytosis. |
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Keywords: | membrane trafficking exocytosis endocytosis patch-clamp membrane capacitance channel cluster |
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