Isolation and characterization of polymorphic microsatellite loci from a dinucleotide-enriched genomic library of spotted maigre (Nibea albiflora) |
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| Authors: | Shichao Xing Changwei Shao Xiaolin Liao Yongsheng Tian Songlin Chen |
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| Institution: | (1) College of Marine Life Sciences, Ocean University of China, Qingdao, 266003, China;(2) Key Lab for Sustainable Utilization of Marine Fisheries Resources, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Nanjing Road 106, Qingdao, 266071, China |
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| Abstract: | In the present study, we reported 13 polymorphic microsatellite loci isolated from a dinucleotide-enriched genomic library
of Nibea albiflora. The number of alleles, observed and expected heterozygosity per locus in 44 individuals ranged from 2 to 13, from 0.0909
to 0.9773 and from 0.0886 to 0.9073, respectively. Three loci significantly deviated from Hardy–Weinberg equilibrium after
Bonferroni correction. Analysis of linkage disequilibrium showed non-significant among the two pairs of loci. As a result,
13 microsatellite loci probably located on different chromosome pairs and these polymorphic microsatellite loci should provide
sufficient level of genetic diversity to investigate the fine-scale population structure and evaluate the breeding strategy
in Nibea albiflora.
Shichao Xing, Changwei Shao contributed equally to this work. |
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| Keywords: | Spotted maigre Nibea albiflora Microsatellite Enriched genomic library |
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