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| 骨髓间充质干细胞移植修复大鼠半横断脊髓损伤 | |
| 引用本文： | 吴金生,董爱萍,王晓萃,魏志新,刘伟光,栾志敏,祝增军. 骨髓间充质干细胞移植修复大鼠半横断脊髓损伤[J]. 中国实验动物学报, 2010, 18(1): 56-59,I0007,I0008 |
| 作者姓名： | 吴金生 董爱萍 王晓萃 魏志新 刘伟光 栾志敏 祝增军 |
| 作者单位： | 1. 山东潍坊医学院附属医院泌尿外科,山东潍坊,261053 2. 潍坊蓝天医院,山东潍坊,261042 3. 潍坊医学院人体解剖学教研室,山东潍坊,261053 4. 潍坊医学院形态学实验室,山东潍坊,261053 |
| 基金项目： | 山东省潍坊市科学技术局资助项目 |
| 摘 要： | 目的初步探讨骨髓间充质干细胞诱导为神经细胞,及其移植对大鼠脊髓半横断损伤神经功能恢复和运动的影响。方法贴壁培养法分离培养大鼠骨髓间充质干细胞(mesenchymal stem cells,MSCs),大鼠脊髓匀浆上清诱导第3代向神经细胞分化,经免疫组化鉴定分化后细胞的性质。制备大鼠半横断脊髓损伤模型,脊髓损伤局部注射BrdU标记诱导后的神经细胞。细胞移植5周后观察移植细胞在脊髓内存活分布情况。结果倒置显微镜下可见MSCs呈纺锤形和多角形,有1~2个核仁,经脊髓匀浆上清诱导后,发出数个细长突起,并交织成网,诱导后的细胞表达Nestin,可推测诱导后的细胞为MSCs源神经细胞。5周后移植的MSCs在宿主损伤脊髓内聚集并存活,表达MAP-2、NF、GFAP与对照组比较有统计学意义(P0.05)。大鼠运动功能较移植前有所改善。结论MSCs经脊髓匀浆上清诱导后移植治疗大鼠半横断脊髓损伤可使运动功能得到改善。 |
| 关 键 词： | 间充质干细胞 脊髓损伤 诱导分化 细胞移植 大鼠 |
Bone Marrow Mesenchymal Stem Cell Transplantation in the Repair of Rat Spinal Cord Hemisection Injury |
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| WU Jin-sheng,DONG Ai-ping,WANG Xiao-cui,WEI Zhi-xin,LIU Wei-guang,LUAN Zhi-min,ZHU Zeng-jun. Bone Marrow Mesenchymal Stem Cell Transplantation in the Repair of Rat Spinal Cord Hemisection Injury[J]. Acta Laboratorium Animalis Scientia Sinica, 2010, 18(1): 56-59,I0007,I0008 | |
| Authors: | WU Jin-sheng DONG Ai-ping WANG Xiao-cui WEI Zhi-xin LIU Wei-guang LUAN Zhi-min ZHU Zeng-jun |
| Affiliation: | WU Jin-sheng~1,DONG Ai-ping~2,WANG Xiao-cui~3,WEI Zhi-xin~4,LIU Wei-guang~1,LUAN Zhi-min~1,ZHU Zeng-jun~1(1.Department of Urology,Affiliated Hospital of Weifang Medical College Weifang 261053,China,2.Lantian Hospital,Weifang 261042,3.Department of Human Anatomy,4.Laboratory of Morphology,Weifang Medical College,Weifang 261053) |
| Abstract: | Objective To investigate the differentiation of bone marrow-derived mesenchymal stem cells into neurons and transplantation of the stem cells to repair rat hemisection spinal cord injury.Methods Adherent culture was used to isolate and culture rat bone marrow mesenchymal stem cells(MSCs).The rat spinal cord homogenate supernatant was used to induce neural differentiation of the 3rd generation cells.The nature of cell differentiation was identified by immunohistochemistry.The rat model of hemisection spinal ... |
| Keywords: | Bone marrow Mesenchymal stem cells Spinal cord injury Induced differentiation Cell transplantation Rat |
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