首页 | 本学科首页   官方微博 | 高级检索  
     


The synthetic precursor specific region of pre-pro-parathyroid hormone forms ion channels in lipid bilayers
Authors:Magdalena T. Tosteson  Michael P. Caulfield  Jay J. Levy  Michael Rosenblatt  Daniel C. Tosteson
Affiliation:(1) Harvard Medical School, 200 Longwood Ave., 02115 Boston, MA, USA;(2) Merck, Sharp & Dohme, West Point, PA, USA
Abstract:
We have used the chemically synthesized sequence of pre-pro-parathyroid hormone and several of its analogues to test the notion that the capacity of amphipathic peptides to aggregate in membranes and form ion-permeable channels correlates with their ability to function as signal sequences for secreted proteins. We found that pre-pro-parathyroid hormone (the signal sequence and pro-region of parathyroid hormone (M)), as well as some of its analogues, forms aggregates of monomers which are ion-permeable. The ion-permeable aggregates (2–3 monomers) formed by (M) are voltage-dependent and are more permeable for cations than for anions. The compounds which formed ion channels in bilayers also acted as potential signal sequences. We conclude that the ability of peptides to form ion-permeable pathways in bilayers may be correlated to their ability to function as signal peptides.
Keywords:ion channel  parathyroid hormone  synthetic precursor  signal sequence
本文献已被 SpringerLink 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号