An immunostimulatory DNA sequence from a probiotic strain of Bifidobacterium longum inhibits IgE production in vitro |
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Authors: | Takahashi Noritoshi Kitazawa Haruki Shimosato Takeshi Iwabuchi Noriyuki Xiao Jin-Zhong Iwatsuki Keiji Kokubo Sadayuki Saito Tadao |
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Institution: | Food Research and Development Laboratory, Morinaga Milk Industry Co., Ltd, Zama, Kanagawa, Japan. no_takah@morinagamilk.co.jp |
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Abstract: | The immunostimulatory oligodeoxynucleotide (ODN) BL07 (5'-GCGTCGGTTTCGGTGCTCAC-3') was identified from the genomic DNA of the probiotic strain Bifidobacterium longum BB536. ODN BL07 stimulated B-lymphocyte proliferation and induced interleukin-12 (IL-12) production in macrophage-like J774.1 cells. ODNs BL07 and BL07S (modified with phosphorothioate backbone) significantly inhibited immunoglobulin E (IgE) production and stimulated interferon-gamma (IFN-gamma) and IL-12 production, but did not affect IL-4 secretion in murine splenic cells of ovalbumin-primed BALB/c mice. These ODNs also significantly inhibited production of IgE in purified murine B cells in the presence of IL-4 and anti-CD40. The results suggest the potential of ODNs BL07 and BL07S in preventing IgE-related immune responses and the possible involvement of ODN BL07 in the antiallergic efficacy of B. longum BB536. |
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Keywords: | probiotics immunostimulatory sequence oligodeoxynucleotides CpG immunoglobulin E cytokine Bifidobacterium longum BB536 |
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