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The fluorescence and bright field microscopic demonstration of cathepsin B in human fibroblasts |
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| Authors: | M. Graf Ursula Leemann F. Ruch P. Sträuli |
| Affiliation: | (1) Division of Cancer Research, Institute of Pathology, University of Zürich, Birchstraße 95, CH-8050 Zürich, Switzerland;(2) Department of General Botany, Swiss Federal Institute of Technology, Universitätsstraße 2, CH-8092 Zürich, Switzerland |
| Abstract: | Summary Cathepsin B was demonstrated cytochemically in human fibroblasts with Z-Ala-Arg-Arg-2-(4-methoxy)naphtylamide as substrate. The enzyme was visualized in the bright field microscope with the diazonium salt Fast Blue B as coupling reagent and in the fluorescence microscope with 5-nitrosalicylaldehyde. With both methods cathepsin B was found in small granules distributed throughout the cytoplasm. |
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