人前列腺生长因子的分离纯化及生物活性鉴定

从人良性增生前列腺组织中经硫酸铵沉淀和肝素－琼脂糖凝胶层析纯化出人前列腺生长因子（ｈＰＧＦ），纯化倍数约１０００倍，ＳＤＳ－ＰＡＧＥ和等电聚焦电泳示分子量约为１７ｋＤ、等电点同标准ｂＦＧＦ，利用分离培养的人前列腺间质成纤维细胞进行活性鉴定，发现以１．３～１．７ｍｏｌ／ＬＮａＣｌ洗脱部分为ｈＰＧＦ，活性最高，对间质成纤维细胞有显著刺激增殖作用。

Purification and Characterization of a Growth Factor from Human Benign Prostatic Hyperplastic Tissue

in order to study the relationship between growth factor and the pathogenesis of benign prostatic hyperplasia,a growth factor was purified about 1 000-fold from human benign hyperplastic prostates by ammonium sulfate precipitation and Heparin-Sepharose chromatography.The fraction containing the majority of mitogenic activity was eluted with 1. 31-7 mol/L NaCl.In SDS/PAGE it showed a molecular weight about 17 kD.The purified growth factor showed potent stimulatory effect on isolated human fetal prostatic fibroblasts.We conclude that this purified growth factor may be a mediator with androgen action.