| 基于粪便DNA的马鹿种群数量和性比 |
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| 引用本文： | 田新民,张明海.基于粪便DNA的马鹿种群数量和性比[J].生态学报,2010,30(22):6249-6254. |
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| 作者姓名： | 田新民 张明海 |
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| 作者单位： | 1. 东北林业大学野生动物资源学院,哈尔滨,150040;牡丹江师范学院生物系,牡丹江,157012
2. 东北林业大学野生动物资源学院,哈尔滨,150040;国家林业局野生动物保护学重点开放性实验室,哈尔滨,150040 |
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| 基金项目： | 国家自然科学基金资助项目(30870309); 黑龙江省自然科学基金重点项目(ZJN-0501); 美国老虎和犀牛基金资助项目(98210-2-G191) |
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| 摘 要： | 为分析黑龙江省完达山林区马鹿种群生存状态,制定科学有效地保护措施,从分子水平研究了种群数量和性比。2006、2007两年冬季跟踪马鹿足迹链,于五泡林场共搜集210份粪便,以成功提取DNA的167份作为分析样本。通过多态性较高的7个微卫星位点进行了基因分型分析,显示167份粪便DNA分属66只个体。基于非损伤性标志重捕法,统计出林场内马鹿数量2a平均47(39—60)只,密度0.302(0.251—0.386)只/km2,与2002年大样方法调查结果相比有减无增。SRY基因性别鉴定显示,种群雌雄性比1.00∶2.00(22♀,44♂),存在较多雄性个体,分析认为偷猎是导致性比失衡的最主要原因。数量的持续下降和性比失衡提示完达山林区马鹿种群数量的恢复需要更好地保护工作。
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| 关 键 词： | 马鹿 粪便DNA 种群数量 性比 |
| 收稿时间： | 2009/10/9 0:00:00 |
| 修稿时间： | 3/1/2010 12:00:00 AM |
Population size and sex ratio of wapiti (Cervus elephus xanthopygus) as revealed
by fecal DNA |
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| Tian Xinmin.Population size and sex ratio of wapiti (Cervus elephus xanthopygus) as revealed
by fecal DNA[J].Acta Ecologica Sinica,2010,30(22):6249-6254. |
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| Authors: | Tian Xinmin |
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| Institution: | TIAN Xinmin1,2,ZHANG Minghai1,3,1 College of Wildlife Resources,Northeast Forestry University,Harbin 150040,China 2 Department of Biology,Mudanjiang Normal College,Mudanjiang 157012,China 3 Key Labortory of Wildlife Conservation,China State Forestry Administration,China |
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| Abstract: | The part of Wandashan Mountains,located in Heilongjiang province,was the area that the density of wapiti was very high. In recent years,the amount of wapiti declined extremely because of many reasons,such as human activity and illegal hunting. For taking conservation measures scientifically and efficiently,we probed into population size and sex ratio, and analyzed its living conditions in this region by molecular method. This method is a good way exactly to obtain the population demography and much informat... |
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| Keywords: | wapiti(Cervus elephus xanthopygus) fecal DNA population size sex ratio |
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