Improved recovery of DNA from polyacrylamide gels after in situ DNA footprinting |
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| Authors: | van Keulen Geertje Meijer Wim G |
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| Affiliation: | Department of Microbiology, Groningen Biotechnology and Biomolecular Science Institute (GBB), University of Groningen, PO Box 14, 9750 AA, Haren, The Netherlands. G.van.Keulen@biol.rug.nl |
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| Abstract: | ![]()
Methods used to date for the isolation of DNA from polyacrylamide gels are elution based, time-consuming and with low yield in DNA. This paper describes an improved system employing polyacrylamide gels made of a meltable matrix. The new system was successfully applied to in situ DNA footprinting following gel retardation assays. |
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