| 葡萄果粒表皮酵母菌多样性研究 |
| |
| 引用本文： | 王 慧,张立强,刘天明,王庆国,谢 荣.葡萄果粒表皮酵母菌多样性研究[J].微生物学报,2008,35(1):10-14. |
| |
| 作者姓名： | 王 慧 张立强 刘天明 王庆国 谢 荣 |
| |
| 作者单位： | 山东轻工业学院 济南 250353;山东轻工业学院 济南 250353;山东轻工业学院 济南 250353;山东轻工业学院 济南 250353;甘肃农业大学农学院 兰州 730070 |
| |
| 基金项目： | 山东省自然科学基金资助项目 (No.Y2005D15) |
| |
| 摘 要： | 从新疆、甘肃、陕西、宁夏、山东主要酿酒葡萄产区收集葡萄果粒并分离得到酵母258株, 利用26S rDNA的D1/D2区序列分析并结合形态学、生理学特征对这些菌株进行了分类学研究, 探讨了这些地区葡萄果粒表皮酵母的物种多样性及其分布。共鉴定出13属26种, 其中优势属为汉逊酵母属Hanseniaspora(5种), 假丝酵母属Candida(4种), 毕赤酵母属Pichia(4种)和伊萨酵母属Issatchenkia(2种)。对分离自不同地域的同种内不同菌株进行了D1/D2序列分析比较, 以探讨不同地理起源地酵母种内序列稳定性及其变异。
|
| 关 键 词： | 酵母菌 多样性 26S rDNA D1/D2 序列分析 |
Species Diversity of Yeasts on the Surface of Grape |
| |
| WANG Hui,ZHANG Li-Qiang,LIU Tian-Ming,WANG Qing-Guo and XIE Rong.Species Diversity of Yeasts on the Surface of Grape[J].Acta Microbiologica Sinica,2008,35(1):10-14. |
| |
| Authors: | WANG Hui ZHANG Li-Qiang LIU Tian-Ming WANG Qing-Guo and XIE Rong |
| |
| Institution: | College of Food and Biologic Engineering, Shandong Institute of Light Industry, Jinan 250353;College of Food and Biologic Engineering, Shandong Institute of Light Industry, Jinan 250353;College of Food and Biologic Engineering, Shandong Institute of Light Industry, Jinan 250353;College of Food and Biologic Engineering, Shandong Institute of Light Industry, Jinan 250353;College of Agronomy, Gansu Agriclutural University, Lanzhou 730070 |
| |
| Abstract: | 258 yeast strains were isolated from the surface of grapes,collected from wine grape cultivation areas in Xinjiang, Gansu, Shaanxi, Ningxia and Shandong Provinces.These strains were identified mainly based on the 26S rDNA D1/D2 domain sequence analysis and morphological and physiological characterization. Among them, 26 species belonging to 13 genera were recognized. The dominant genera identified were Hanseniaspora (5 species), Candida (4 species), Pichia (4 species) and Issatchenkia(2 species). D1/D2 sequence variation were compared among the strains of the same species from different areas. |
| |
| Keywords: | grape yeast species diversity 26S rDNA D1/D2 domain sequence analysis |
|
| 点击此处可从《微生物学报》浏览原始摘要信息 |
| 点击此处可从《微生物学报》下载免费的PDF全文 |
|
