High throughput culture and gametogenesis induction of <Emphasis Type="Italic">Laminaria japonica</Emphasis> gametophyte clones |
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Authors: | Quan Sheng Zhang Shan Cun Qu Yi Zhou Cong Shi Ju Luo Xue Xi Tang |
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Institution: | (1) College of Marine Life Sciences, Ocean University of China, Qingdao, 266003, People’s Republic of China;(2) Ocean School, Yantai University, Yantai, 264005, People’s Republic of China;(3) Shandong Oriental Ocean Sci-tech Co., Ltd., Yantai, 264005, People’s Republic of China |
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Abstract: | In anticipation of the application of a new sporeling-raising method using gametophyte clones to Laminaria commercial cultivation in China, techniques of mass culture and gametogenesis induction of L. japonica gametophyte clones were developed, as a mass of fertile gametophytes is a prerequisite for sporeling-raising with the new
method. Gametophyte clones which were subjected to fed-batch culture exhibited a classical logistic growth curve. Growth rates
decreased gradually after 2 months of culture, and were negatively correlated to cell density. UNOVA also showed that only
cell density has a significant effect on the growth of gametophyte clones under the experimental conditions. Based on the
dynamics models revealed, a culture strategy only directed at the control of cell density was adopted. By this strategy, a
total of 36 kg wet weight from an initial weight of 0.75 kg was achieved after 3 months culture in 100 20-L bottles. The final
average density reached 24 g L−1. For the subsequent gametogenesis induction, amplificatory male and female gametophyte clones were cut, mixed and cultured
in bottles under the same conditions used in amplification except for a change of photoperiod from continuous irradiance to
10 h light: 14 h dark cycle. Egg discharge occurred 10 days after the mixed culture and increased gradually with the culture
duration. Most gametophytes gave rise to sporophytes 20 days after induction. Large-scale culture of gametophyte clones and
gametogenesis induction for commercial cultivation in 2003–2005 have been conducted successfully. |
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Keywords: | Gametophyte clone Growth dynamics Induction of gametogenesis Laminaria japonica Large-scale culture Vegetative growth |
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