Effects of puromycin aminonucleoside on excision repair and recombination repair inescherichia coli |
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Authors: | Dr Aris S Sideropoulos |
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Institution: | (1) Department of Biological Sciences, Duquesne University, PA 15282 Pittsburgh, Pennsylvania, USA |
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Abstract: | Ultraviolet (UV) lethality was increased when puromycin aminonucleoside (PAN) (3.0 mM) was added to the postirradiation medium ofEscherichia coli strains. The extent of repair inhibition differed greatly for strains WP-2hcr
+, B/r()hcr
+, WP-2hcr
–, and Bs-1hcr
–. The interaction between PAN and UV was synergistic in thehcr
+ strains. PAN enhanced UV lethality in strain B/r () to a greater degree than in WP-2hcr
+. There was no UV lethality enhancement by PAN (3.0 mM) in thehcr
– strains, but the interaction of PAN (8.0 mM) with UV was synergistic. PAN decreased plaque formation of T1 UV-irradiated phage plated onE. coli Bhcr
+ but had no effect on phage plated on Bs-1 or WP-2hcr
– strains. These results suggest that PAN interferes with thehcr function in UV-irradiated bacteria. |
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Keywords: | |
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