DSA技术在恒河猴脑脊液动态采集中应用

探索数字减影血管造影(digital subtraction angiography,DSA)插管技术在恒河猴(Macaca mulatta)脑脊液动态采集中的应用。将28只恒河猴作为实验动物,随机分为实验组和对照组,每组14只,经左前肢静脉推注3%戊巴比妥钠溶液全麻后,常规剔除背部脊柱周围体毛、消毒,实验组从穿刺开始就在DSA技术下行腰椎间隙穿刺,对照组直接行腰椎间隙穿刺,将硬膜外麻醉导管经腰椎蛛网膜下腔送至枕大池并埋置泵。对不同穿刺方法的结果数据进行χ2检验;神经功能参照改良Tarlov评分标准,切口采用改良的切口愈合等级评分标准。实验组经腰椎间隙穿刺蛛网膜下腔全部成功;对照组8只穿刺成功,6只穿刺失败;所有动物埋置泵均能从泵中采集到无色透明的脑脊液;术后恒河猴均于当天恢复正常。在DSA技术下经腰椎间隙穿刺蛛网膜下腔并埋置泵,成功率高,操作简便、安全性好,可行性强;通过埋置的泵采集脑脊液,快速省时,可实现在动物清醒时、全生理状态下对脑脊液进行动态多次采集,有助于提高非人灵长类实验动物的福利,由于采样导管置于枕大池,采集到的脑脊液样品质量有保证,可用于研究缺血性脑卒中发生机制,满足检验需要,具有一定的实用价值。

Application of DSA Technique in Dynamic Acquisition of Cerebrospinal Fluid in Rhesus Macaque

The aim of the present study was to explore the application of digital subtraction angiography(DSA) intubation technique in the dynamic acquisition of cerebrospinal fluid in Ganges River Monkey(Macaca mulatta). A total of 28 monkeys were randomly divided into two groups: experimental group and control group, 14 individuals each. Each monkey was injected with 3% pentobarbital sodium solution through left anterior limb vein for general nesthesia, and then hairs on the back around the spine were eliminated after disinfection. In the experimental group, lumbar puncture was started with the DSA targeting, and the control group lumbar puncture was directly performed. The epidural anesthesia catheter was sent from thelumbar subarachnoid space to the occipital cistern, in which the pump was buried for all 28 monkeys. The data were analyzed with Chi-square test. Modified Tarlov reference criteria were used for neural function evaluation, and a modified incision healing grade standard was also used for healing evaluation, using Spss17.0 software for data analysis. In the control group, we obtained 8 cases of success and 6 cases of failure,with no cerebrospinal fluid outflow in all animals. In the experimental group, the lumbar puncture was successful. All the pumps were able to collect colorless and transparent cerebrospinal fluid. After surgery, all the Ganges River Monkeys were returned to normal on the same day. With DSA intubation technique, the operation is simple, safe, and feasible with high success of erebrospinal fluid collection. By embedding the pump, quick and repeated acquisition of cerebrospinal fluid can be realized when the animals are awake and in full physiological state, which will help to improve the welfare of nonhuman experimental primate animals.Sampling catheters are placed in the cisterna, which will guarantee the quality of collected cerebrospinal fluid,the convenience of studying pathogenesis of ischemic stroke, thus this approach has a certain practical value.