Sources of uncertainty in the quantification of genetically modified oilseed rape contamination in seed lots |
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Authors: | Graham S Begg Danny W Cullen Pietro P M Iannetta Geoff R Squire |
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Institution: | (1) Scottish Crop Research Institute, Invergowrie, Dundee, DD2 5DA, UK |
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Abstract: | Testing of seed and grain lots is essential in the enforcement of GM labelling legislation and needs reliable procedures for
which associated errors have been identified and minimised. In this paper we consider the testing of oilseed rape seed lots
obtained from the harvest of a non-GM crop known to be contaminated by volunteer plants from a GM herbicide tolerant variety.
The objective was to identify and quantify the error associated with the testing of these lots from the initial sampling to
completion of the real-time PCR assay with which the level of GM contamination was quantified.
The results showed that, under the controlled conditions of a single laboratory, the error associated with the real-time PCR
assay to be negligible in comparison with sampling error, which was exacerbated by heterogeneity in the distribution of GM
seeds, most notably at a small scale, i.e. 25 cm3. Sampling error was reduced by one to two thirds on the application of appropriate homogenisation procedures. |
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Keywords: | Genetic modification Seed lot Sampling error Measurement error Heterogeneity Real-time PCR |
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