| 抑制ATR基因可提高HeLa细胞对烷化剂的敏感性 |
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| 引用本文： | 王颖丽,让文亮,张雪,檀英霞,李素波,鲍国强,高红伟,宫锋,季守平.抑制ATR基因可提高HeLa细胞对烷化剂的敏感性[J].生物技术通讯,2013(4):501-503,535. |
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| 作者姓名： | 王颖丽 让文亮 张雪 檀英霞 李素波 鲍国强 高红伟 宫锋 季守平 |
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| 作者单位： | 军事医学科学院野战输血研究所,北京100850 |
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| 基金项目： | 国家自然科学基金（30671995） |
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| 摘 要： | 目的：探讨ATR基因的表达对HeLa细胞对烷化剂敏感性的影响。方法：采用RNA干扰技术抑制HeLa细胞ATR基因的表达，观察ATR被阻断后HeLa细胞对烷化剂敏感性的变化，从而确定ATR基因的作用。结果：筛选到表达针对ATR基因的短发夹RNA（shRNA）阳性克隆，Western印迹结果显示ATR基因表达受到明显抑制；HeLa细胞对烷化剂的敏感性实验提示，ATR shRNA^＋HeLa细胞对烷化剂的敏感性明显增强。结论：抑制ATR基因可明显提高HeLa细胞对烷化剂的敏感性。
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| 关 键 词： | ATR基因 烷化剂 HeLa细胞 |
Expression Inhibition of ATR Gene Improve the Susceptibility of HeLa Cells to Alkylating Agents |
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| WANG Ying-Li,RANG Wen-Liang,ZHANG-Xue,TAN Ying-Xia,LI Su-Bo,BAO Guo-Qiang,GAO Hong-Wei,GONG Feng,JI Shou-Ping.Expression Inhibition of ATR Gene Improve the Susceptibility of HeLa Cells to Alkylating Agents[J].Letters in Biotechnology,2013(4):501-503,535. |
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| Authors: | WANG Ying-Li RANG Wen-Liang ZHANG-Xue TAN Ying-Xia LI Su-Bo BAO Guo-Qiang GAO Hong-Wei GONG Feng JI Shou-Ping |
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| Institution: | ( Beijing Institute of Transfusion Medicine, Beijing 100850) |
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| Abstract: | Objective： To investigate the effect of the expression of ATR gene on HeLa cells＇ susceptibility to al-kylating agents. Methods： RNA interference technology was employed to suppress the expression of ATR, then the role of ATR in HeLa cells＇ anti-alkylation ability was inspected. Results： A positive clone of siRNA against ATR gene was successfully screened and it can strikingly inhibit the expression of ATR in HeLa cells by Western blot analysis, and then the ability of HeLa cells to resist alkylating agents was significantly attenuated. Conclusion： The inhibition of the expression of ATR gene can significantly improve the HeLa cells＇ susceptibility to alkylating agents. |
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| Keywords: | ATM-Rad3-related gene alkylating agent HeLa cells |
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