| 野大麦幼穗原生质体的分离和培养 |
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| 引用本文： | 李彦舫,程肖蕊,张亚兰,庞劲松.野大麦幼穗原生质体的分离和培养[J].植物学通报,1999,16(1):67-71. |
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| 作者姓名： | 李彦舫 程肖蕊 张亚兰 庞劲松 |
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| 作者单位： | 解放军农牧大学生物教研室 |
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| 摘 要： | 以野大麦（Ｈｏｒｄｅｕｍｂｒｅｖｉｓｕｂｕｌａｔｕｍ）的幼穗诱导愈伤组织，建立悬浮细胞系，利用胚性悬浮细胞系在不同的酶解条件和不同的酶解液中分离原生质体并进行培养，获得了细胞分裂，形成细胞团。
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| 关 键 词： | 野大麦，原生质体，细胞分裂 |
PROTOPLAST ISOLATION AND CULTURE OF YOUNG INFLORESCENCES OF WILD BARLEY |
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| LI Yan,Fang,CHENG Xiao,Rui,ZHANG Ya,Lan,PANG Jin,Song.PROTOPLAST ISOLATION AND CULTURE OF YOUNG INFLORESCENCES OF WILD BARLEY[J].Chinese Bulletin of Botany,1999,16(1):67-71. |
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| Authors: | LI Yan Fang CHENG Xiao Rui ZHANG Ya Lan PANG Jin Song |
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| Abstract: | Embryogenic suspension cell line was established from calli which were induced from young inflorescences of wild barley (Hordeum brevisubulatum. After isolated from the suspension cell line under different conditions and enzyme solutions, protoplasts were cultured, cell division and cell cluster were observed. |
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| Keywords: | Wild barley Protoplast Cell division |
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