首页 | 本学科首页   官方微博 | 高级检索  
     


Extracellular lipid droplets promote hemozoin crystallization in the gut of the blood fluke Schistosoma mansoni
Authors:Corrêa Soares Juliana B R  Maya-Monteiro Clarissa M  Bittencourt-Cunha Paula R B  Atella Geórgia C  Lara Flávio A  d'Avila Joana C P  Menezes Diego  Vannier-Santos Marcos A  Oliveira Pedro L  Egan Timothy J  Oliveira Marcus F
Affiliation:Instituto de Bioquímica Médica, Programa de Biologia Molecular e Biotecnologia, Universidade Federal do Rio de Janeiro, Cidade Universitária, Rio de Janeiro, RJ, Brazil.
Abstract:Hemozoin (Hz) is a heme crystal produced upon hemoglobin digestion as the main mechanism of heme disposal in several hematophagous organisms. Here, we show that, in the helminth Schistosoma mansoni, Hz formation occurs in extracellular lipid droplets (LDs). Transmission electron microscopy of adult worms revealed the presence of numerous electron-lucent round structures similar to LDs in gut lumen, where multicrystalline Hz assemblies were found associated to their surfaces. Female regurgitates promoted Hz formation in vitro in reactions partially inhibited by boiling. Fractionation of regurgitates showed that Hz crystallization activity was essentially concentrated on lower density fractions, which have small amounts of pre-formed Hz crystals, suggesting that hydrophilic-hydrophobic interfaces, and not Hz itself, play a key catalytic role in Hz formation in S. mansoni. Thus, these data demonstrate that LDs present in the gut lumen of S. mansoni support Hz formation possibly by allowing association of heme to the lipid-water interface of these structures.
Keywords:Hemozoin   Heme   Lipid droplets   Schistosoma mansoni
本文献已被 ScienceDirect PubMed 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号