Phylogenetic Characterization of Archaea in Saltpan Sediments |
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Authors: | Nasier Ahmad Sarojini Johri Phalisteen Sultan Malik Z Abdin Ghulam N Qazi |
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Institution: | (1) Biotechnology Division, Indian Institute of Integrative Medicine (Council of Scientific and Industrial Research), Canal Road, Jammu-Tawi, 180001, India;(2) Microbiology and Mycology Division, Indian Institute of Integrative Medicine (CSIR) Br, Srinagar, 190005, India;(3) Biotechnology Department, Faculty of Science, Jamia Hamdard University, Hamdard Nagar, New Delhi, 110062, India |
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Abstract: | A study was undertaken to investigate the presence of archaeal diversity in saltpan sediments of Goa, India by 16S rDNA-dependent
molecular phylogeny. Small subunit rRNA (16S rDNA) from saltpan sediment metagenome were amplified by polymerase chain reaction
(PCR) using primers specific to the domain archaea. 10 unique phylotypes were obtained by PCR based RFLP of 16S rRNA genes
using endonuclease Msp 1, which was most suitable to score the genetic diversity. These phylotypes spanned a wide range within the domain archaea
including both crenarchaeota and euryarcheaota. None of the retrieved crenarchaeota sequences could be grouped with previously
cultured crenarchaeota however; two sequences were related with haloarchaea. Most of the sequences determined were closely
related to the sequences that had been previously obtained from metagenome of a variety of marine environments. The phylogenetic
study of a site investigated for the first time revealed the presence of low archaeal population but showed yet unclassified
species, may specially adapted to the salt pan sediment of Goa. |
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Keywords: | Small subunit rRNA Phylogeny Archaea Saltpan Hypersaline environment |
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